The replication of Study 51: [Crasta et al., 2012][1] was [not pursued][2]; however, this project contains all information pertaining to the attempted replication of key experiments from this paper. It includes the detailed protocols, including reagents and author clarifications where they are available. <br> **Original paper:** <br> Crasta K., Ganem N.J., Dagher R., Lantermann A.B., Ivanova E.V., Pan Y., Nezi L., Protopopov A., Chowdhury D., Pellman D. <a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3271137/">DNA breaks and chromosome pulverization from errors in mitosis.</a> Nature. 2012 Feb 2;482(7383):53-8. doi: 10.1038/nature10802. <br> **Original paper abstract:**<br> The involvement of whole-chromosome aneuploidy in tumorigenesis is the subject of debate, in large part because of the lack of insight into underlying mechanisms. Here we identify a mechanism by which errors in mitotic chromosome segregation generate DNA breaks via the formation of structures called micronuclei. Whole-chromosome-containing micronuclei form when mitotic errors produce lagging chromosomes. We tracked the fate of newly generated micronuclei and found that they undergo defective and asynchronous DNA replication, resulting in DNA damage and often extensive fragmentation of the chromosome in the micronucleus. Micronuclei can persist in cells over several generations but the chromosome in the micronucleus can also be distributed to daughter nuclei. Thus, chromosome segregation errors potentially lead to mutations and chromosome rearrangements that can integrate into the genome. Pulverization of chromosomes in micronuclei may also be one explanation for ‘chromothripsis’ in cancer and developmental disorders, where isolated chromosomes or chromosome arms undergo massive local DNA breakage and rearrangement. [1]: http://www.nature.com/nature/journal/v482/n7383/full/nature10802.html [2]: https://osf.io/ucpye/