## Registered report: *Fusobacterium nucleatum* infection is prevalent in human colorectal carcinoma <br> **Abstract** The [Reproducibility Project: Cancer Biology][1] seeks to address growing concerns about reproducibility in scientific research by conducting replications of selected experiments from a number of high-profile papers in the field of cancer biology. The papers, which were published between 2010 and 2012, were selected on the basis of [citations and Altmetric scores][2] ([Errington et al., 2014][3]). This Registered Report describes the proposed replication plan of key experiments from '*Fusobacterium nucleatum* infection is prevalent in human colorectal carcinoma' by Castellarin and colleagues published in *Genome Research* in 2012 ([Castellarin et al., 2012][4]). The experiment to be replicated is reported in Figure 2. Here, Castellarin and colleagues performed a metagenomic analysis of colorectal carcinoma (CRC) to identify potential associations between inflammatory microorganisms and gastrointestinal cancers. They conducted quantitative real-time PCR on genomic DNA isolated from tumor and matched normal biopsies from a patient cohort and found that the overall abundance of *Fusobacterium* was 415 times greater in CRC versus adjacent normal tissue. These results confirmed earlier studies and provide evidence for a link between tissue-associated bacteria and tumorigenesis. The Reproducibility Project: Cancer Biology is a collaboration between the [Center for Open Science][5] and [Science Exchange][6] and the results of the replications will be published in [*eLife*][7]. ---------- ### Contents This component contains additional materials relating to the Registered Report for Study 50: Castellarin et al., 2012 (*Genome Research*). The Registered Report can be found [here][8]. **Registered Report Initial Draft**: - This document represents the first draft of the selected experimental protocols. It contains the information that the Core team, with help from scientist volunteers, was able to glean from the original publication and its supplemental materials before the original authors were contacted for their input. - [Study 50 Registered Report Initial Draft][9] **Data Estimation**: - When original data was unable to be obtained, or original qualitative data was quantified (for example, Western blots), the estimated data is contained here. Examples include densitometric quantification of Western blot bands, or estimations of means and variances from published graphs. - [Study 50 Data Estimation][10] **Power Calculations**: - These documents contain details about how power calculations were performed, and include spreadsheets documenting statistical tests as well as any PRISM files, R scripts or additional materials used. - [Study 50 power calculations][11] - [Study 50 Figure 2 ratio t-test.R][12] [1]: https://osf.io/e81xl/wiki/home/ [2]: https://osf.io/e81xl/wiki/studies/ [3]: https://elifesciences.org/content/3/e04333 [4]: http://genome.cshlp.org/content/22/2/299 [5]: https://cos.io/ [6]: https://www.scienceexchange.com/ [7]: https://elifesciences.org/ [8]: https://elifesciences.org/content/5/e10012 [9]: https://osf.io/v6t3n/ [10]: https://osf.io/sf74z/ [11]: https://osf.io/kxstg/ [12]: https://osf.io/bjgn5/