**Figure 2. Expression of PREX2 isoforms.** NRAS<sup>G12D</sup> melanocytes were transduced to express GFP or PREX2 isoforms (PREX2<sup>WT</sup>, PREX2<sup>G844D</sup>, PREX2<sup>Q1430*</sup>). (**A**) Representative Western blot
using an anti-V5 antibody (top panel) or an anti-alpha-Tubulin antibody (bottom panel). (**B**) Relative protein expression (V5/Tubulin) of ectopically expressed PREX2 isoforms are presented for each stable cell line and normalized to PREX2<sup>WT</sup> cells made during the original study. Two-way ANOVA main effect of PREX2 isoform (*F*(2,12) = 4.608, *p* = .033), the main effect of study (*F*(1,12) = 8.701, *p* = .012), and isoform:study interaction (*F*(2,12) = 7.52, *p* = .008). (**C**) Representative Western blot using an anti-PREX2 antibody (top panel) or an anti-alpha-Tubulin antibody (bottom panel). (**D**) Relative protein expression (PREX2/Tubulin) of ectopically expressed PREX2 isoforms are presented for each stable cell line and normalized to PREX2<sup>WT</sup> cells generated during the original study. Two-way ANOVA main effect of PREX2 expression (*F*(3,16) = 15.033, *p* = 6.47x10<sup>-5</sup>), the main effect of study (*F*(1,16) = 13.08, *p* = .002), expression:study interaction (*F*(3,16) = 4.50, *p*= .018).
Access the [analysis scripts][1] for the statistical tests related to this figure. These scripts will call the [data][2] directly from the OSF.
[1]: https://osf.io/w52c7/files/
[2]: https://osf.io/nm2jv/files/
