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Full instructions provided in the following HardwareX paper: Kehl, F., Cretu, V.F., Willis, P.A., Open-Source Lab Hardware: A Versatile Microfluidic Control and Sensor Platform, *HardwareX* **2021**. **Abstract:** Here we describe a completely integrated and customizable microfluidic control and sensing architecture that can be readily implemented for laboratory or portable chemical or biological control and sensing applications. The compact platform enables control of 32 solenoid valves, a multitude of pumps and motors, a thermo-electric controller, a pressure controller, and a high voltage power supply. It also features two temperature probe interfaces, one relative humidity and ambient temperature sensor, two pressure sensors, and interfaces to an electrical conductivity sensor, flow sensor, and a bubble detector. The platform can be controlled via an onboard microcontroller and requires no proprietary software. **Design files description:** - MCSP PCB V1.0.sch: Autodesk Eagle schematic file. The PCB schematic can be viewed and edited if desired. - MCSP PCB V1.0.brd: Autodesk Eagle board file. Needed to produce the PCB. - MCSP PCB V1.0.lbr: Autodesk Eagle part library for the PCB. - MCSP_Firmware_V1.0.zip: Contains the *.ino, *.cpp, and *.h files to program the Teensy controller. - MCSP PCBA Parasolid.x_t: 3D model of the PCB assembly. - MCSP Enclosure Box.stl: STL-File to 3D-print the enclosure box. - MCSP Enclosure Lid.stl: STL-File to 3D-print the enclosure lid.
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