Figure 1. Isocitrate-dependent NADPH production and alpha-ketoglutarate-dependent NADPH consumption of wild-type or R172 mutant IDH2. Number of mice: (A) A549 tumors: n=14 (vehicle), n=13 (cimetidine), n=4 (doxorubicin) (B) ACHN tumors: n=15 (vehicle), n=15 (cimetidine). Two-tailed Welch’s t-test on Day 11 relative volumes between vehicle or doxorubicin treated A549 tumors; t (6.73) = 2.68, uncorrect p = .0325 with a priori alpha level = .0167; (Bonferroni corrected p = .0976). Two-way ANOVA interaction between cell line (A549 or ACHN) and treatment (vehicle or cimetidine) on natural log transformed Day 11 relative volumes; F (1,53) = 3.88, p = .054. Pairwise contrast between A549 tumors treated with vehicle or cimetidine; t (53) = 2.16, uncorrected p = .035 with a priori alpha level = .0167; (Bonferroni corrected p = .105). Pairwise contrast between ACHN tumors treated with vehicle or cimetidine; t (53) = -0.58, uncorrected p = .562 with a priori alpha level = .0167; (Bonferroni corrected p > .99).
Access analysis script for the statistical tests related to this figure. This script will call the data directly from the OSF.
