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In this work, we performed multi-omics analysis of lignocellulosic carbon utilization in R. toruloides and reconstructed the genome-scale metabolic network of R. toruloides. The metabolic network model developed in this study was reconstructed and manually curated reproducibly using electronic notebooks, and validated against multi-omics and high-throughput growth data, significantly expanding the breadth and depth of metabolic coverage from previously published models.
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