Target of replication: *A priori* experimental protocols, materials, and confirmatory analysis plans were peer reviewed, [pre-registered][1], and published by [*eLife*][2].
This component includes all primary and QC data for the replication of [Garnett et al. 2012][3]. This experiment analyzed Olaparib sensitivity in cells transformed with the EWS-FLI1 rearrangement.
### Contents ###
[Figures component][4] contains a visualization of the results. These figures are the same as can be found in the Replication Study and include:
- **Figure 2. Olaparib sensitivity in cells transformed with the EWS-FLI1 rearrangement.**, which displays the results of the cell viability assay.
- **Figure 2 - figure supplement 1. Population doubling time of cells and confirmation of EWS-FLI1 rearrangement**, whicih displays the relative viability of untreated and vehicle control treated cells used to determine the population doubling times and the relative expression levels of EWS-FLI1 and Actb were determined by qRT-PCR for each cell line.
[Data Component][5], contains data generated from the experimental work. Csv files containing the data used in the analysis of protocol 2, which assesses Olaparib sensitivity in cells transformed with the EWS-FLI1 rearrangement, can be found [here][6] for the qPCR data used in analysis and [here][7] for the viability data used in analysis.
[Statistical Analysis Component][8], contains the R scripts used for the analysis.
[Methods Component][9] includes all methods, information about equipment used, and lab notes necessary for completing this protocol.
[Cell line quality Control data][10] includes results confirming the cell line was free of mycoplasma contamination as well as STR DNA profiling.
[1]: https://osf.io/j9bnk/
[2]: https://elifesciences.org/articles/13620
[3]: https://www.nature.com/articles/nature11005
[4]: https://osf.io/4ccz9/wiki/home/
[5]: https://osf.io/5j89g/wiki/home/
[6]: https://osf.io/k95ag/
[7]: https://osf.io/dqy7q/
[8]: https://osf.io/a69tk/wiki/home/
[9]: https://osf.io/egzwh/wiki/home/
[10]: https://osf.io/pfh3x/wiki/home/
