@[toc](Visualizations)
@[osf](2a3zd)
#### Movie S1 ####
Fluorescent beads on the surface of a glass coverslip, embedded in lens immersion oil, axially scanned from $z_f = −500$ nm to $z_f = 500$ nm. Colorbar: photons; scale bar: 2 µm.
@[osf](3bvx2)
#### Movie S2 ####
Three-dimensional view and cross-sectional images of Nile red localizations on the 1000 nm-radius lipid-coated sphere in Figure 3. Localizations are depicted as points at left and as line segments matching the measured molecule orientations at right. Color scale: (left) polar angle θ in deg, (right) azimuthal angle ϕ in deg.
@[osf](4f67w)
#### Movie S3 ####
Three-dimensional view of Nile red localizations on 350 nm-radius lipid-coated spheres incubated without (day 0) and with Aβ42 (Figure 4). Color scale: azimuthal angle ϕ in deg.
@[osf](g9d3y)
#### Movie S4 ####
Two-dimensional (xy) view of merocyanine 540 localizations along the HEK-293T cell membrane in Extended Data Figure 5a, and a sliding window showing yz cross-sections. Localizations are depicted as points at left and as line segments matching the measured molecule orientations at right. Color scale: axial position z in nm.
@[osf](e9jkw)
#### Movie S5 ####
Three-dimensional view of merocyanine 540 localizations along the HEK-293T cell membrane in Extended Data Figure 5c-e. Localizations are depicted as line segments matching the measured molecule orientations. Color scale: (left) polar angle ϕ in deg, (middle) wobble solid angle Ω in sr, (right) azimuthal angle ϕ in deg.
@[osf](j6dzg)
#### Movie S6 ####
Blinking merocyanine 540 molecules along the HEK-293T cell in Figure 5a. Dashed lines represent where the cell is in contact with coverslip (bottom-right area in each channel). The nominal focal plane is placed at $z_f = 1200$ nm. Colorbar: photons; scale bar: 5 µm.
@[osf](ew3nk)
#### Image S1 ####
[High-resolution image][1] of merocyanine 540 localizations on the HEK-293T cell in Figure 5.
Lines are oriented and color-coded according to the azimuthal angle φ. Their lengths are
proportional to the projection of the unit vector μ into the xy plane and are equal to $100 \sin \theta$
nm.
@[osf](2jymw)
#### Image S2 ####
[High-resolution image][2] of merocyanine 540 localizations on the HEK-293T cell in Extended
Data Figure 5. Lines are oriented and color-coded according to the azimuthal angle φ. Their
lengths are proportional to the projection of the unit vector μ into the xy plane and are
equal to $100 \sin \theta$
nm.
@[osf](unkbh)
#### Image S3 ####
[High-resolution image][3] of NR4A localizations on the HEK-293T cell in Extended Data Figure
6a,b. Lines are oriented and color-coded according to the azimuthal angle φ. Their lengths
are proportional to the projection of the unit vector μ into the xy plane and are equal to
$100 \sin \theta$
nm.
@[osf](6wzjv)
#### Image S4 ####
[High-resolution image][4] of NR4A localizations on the HEK-293T cell in Extended Data Figure
6c,d. Lines are oriented and color-coded according to the azimuthal angle φ. Their lengths
are proportional to the projection of the unit vector μ into the xy plane and are equal to
$100 \sin \theta$
nm.
[1]: https://osf.io/ew3nk
[2]: https://osf.io/2jymw
[3]: https://osf.io/unkbh
[4]: https://osf.io/6wzjv
