Target of replication: *A priori* experimental protocols, materials, and confirmatory analysis plans were peer reviewed, [pre-registered][1], and published by [*eLife*][2]. This component includes all data, analysis scripts, and methods for the replication of the experiment reported in Figure 2 of [Castellarin et al., 2012][3]. This experiment used qPCR to investigate the level of _Fusobacterium nucleatum_ in colorectal carcinoma, adjacent normal, and matched normal tissues. <br> ### Contents [Figures component][4] contains a visualization of replication results. These figures are the same as can be found in the Replication Study and include Figure 1 displaying mean _Fusobacterium nucleatum_ DNA levels in CRC tissue normalized to adjacent normal tissue. [Statistical Analyses component][5] contains R scripts that were used for the analysis. [Data component][6] contains data generated from the experimental work and files used in the analysis and generation of figures, which are in .csv format. [Methods component][7] contains methodology details for this replication attempt which are described in the [Registered Report][8]. [Clinical samples quality control data][9] includes patient phenotypes and genomic DNA concentration and purity. [1]: https://osf.io/2wmfb/ [2]: https://elifesciences.org/content/5/e10012 [3]: http://genome.cshlp.org/content/22/2/299 [4]: https://osf.io/vf6zs/wiki/home [5]: https://osf.io/9gjyz/wiki/home [6]: https://osf.io/teyjx/wiki/home [7]: https://osf.io/rj9pq/wiki/home [8]: https://elifesciences.org/content/5/e10012 [9]: https://osf.io/zt2h8/wiki/home