Nanopore sequencing undergoes catastrophic sequence failure at inverted duplicated DNA sequences

doi:None

Title	Authors

Home

Data for: **Nanopore sequencing undergoes catastrophic sequence failure at inverted duplicated DNA sequences** Pieter Spealman, Jaden Burrell, and David Gresham Inverted duplicated sequences are a common feature of structural variants (SVs) and copy number variants (CNVs). Analysis of CNVs containing inverted duplicated sequences using nanopore sequencing identified recurrent aberrant behavior characterized by incorrect and low confidence base calls that result from a systematic elevation in the current recorded by the sequencing pore. The coincidence of inverted duplicated sequences with catastrophic sequence failure suggests that secondary DNA structures may impair transit through the nanopore.

OSF does not support the use of Internet Explorer. For optimal performance, please switch to another browser.

This website relies on cookies to help provide a better user experience. By clicking Accept or continuing to use the site, you agree. For more information, see our Privacy Policy and information on cookie use.

Start managing your projects on the OSF today.

Free and easy to use, the Open Science Framework supports the entire research lifecycle: planning, execution, reporting, archiving, and discovery.

Create an Account Learn More Hide this message

Main content

Home

Menu

Start managing your projects on the OSF today.

Main content

Links to this project

Home

Menu

Add new wiki page

Page permissions have changed

Wiki page deleted

Connected to the collaborative wiki

Connecting to the collaborative wiki

Collaborative wiki is unavailable

Browser unsupported

Start managing your projects on the OSF today.