## Registered report: Tumour vascularization via endothelial differentiation of glioblastoma stem-like cells <br> **Abstract:** The [Reproducibility Project: Cancer Biology][1] seeks to address growing concerns about reproducibility in scientific research by conducting replications of 50 papers in the field of cancer biology published between 2010 and 2012. This Registered report describes the proposed replication plan of key experiments from ‘Tumour vascularization via endothelial differentiation of glioblastoma stem-like cells’ by Ricci-Vitiani and colleagues, published in *Nature* in 2010 ([Ricci-Vitiani et al., 2010][2]). The experiments that will be replicated are those reported in Figure 4B and Supplementary Figure 10B ([Ricci-Vitiani et al., 2010][3]), which demonstrate that glioblastoma stem-like cells can derive into endothelial cells, and can be selectively ablated to reduce tumor progression in vivo, and Supplementary Figures S10C and S10D ([Ricci-Vitiani et al., 2010][4]), which demonstrate that fully differentiated glioblastoma cells cannot form functionally relevant endothelium. The Reproducibility Project: Cancer Biology is a collaboration between the [Center for Open Science][5] and [Science Exchange][6], and the results of the replications will be published by [*eLife*][7]. ---------- ### Contents This component contains additional materials relating to the Registered Report for Study 5: Ricci-Vitiani et al. 2010, Nature. The Registered Report can be found [here][8]. ---------- **Registered Report Initial Draft**: - This document represents the first draft of the selected experimental protocols. It contains the information that the Core team, with help from scientist volunteers, was able to glean from the original publication and its supplemental materials before the original authors were contacted for their input. - <a href="https://osf.io/kushg/">Study 5 Registered Report Initial Draft</a> **Power Calculations**: - These documents contain details about how power calculations were performed, and include spreadsheets documenting statistical tests as well as any PRISM files, R scripts or additional materials used. - <a href="https://osf.io/4m6qv/">Study 5 power calculations.xlsx</a> - <a href="https://osf.io/8sbn7/">Study 5 ANOVA calculations.pzfx</a> - <a href="https://osf.io/8ys3x/">Study 5 Protocol 3 Figure S10C 1-way ANOVA.R</a> [1]: https://osf.io/e81xl/wiki/home/ [2]: https://doi.org/10.1038/nature09557 [3]: https://doi.org/10.1038/nature09557 [4]: https://doi.org/10.1038/nature09557 [5]: http://centerforopenscience.org/ [6]: https://www.scienceexchange.com/ [7]: https://elifesciences.org/ [8]: http://elifesciences.org/content/4/e04363