This project contains all information pertaining to the replication of experiments from “Tumour vascularization via endothelial differentiation of glioblastoma stem-like cells” published in *Nature* in 2010 ([Ricci-Vitiani et al., 2010][1]). Experimental work began although not all experiments were completed. The completed experiments were included in the summary paper "Experiments from unfinished Registered Reports in the Reproducibility Project: Cancer Biology" ([Errington et al., 2021][2]). <br> Also, explore the Registered Report and materials related to the Registered Report [here][3]. <br> **Data and Material Availability**: All other associated data, protocols, analysis scripts, and other digital materials are available within this OSF project. **Experiments replicated**: Reproduce and explore the figures, analyses, data, and methods generated in this replication attempt. - [Evaluation of Tie2 expression in various cell lines using qPCR][4] - Replication of Figure S11C of Ricci-Vitiani et al., 2010 - [Lentiviral infection of glioblastoma cells and stable cell generation][5] - Attempt of replication of Figures 4B and S10B of Ricci-Vitiani et al., 2010 **Meta-analysis**: As a measure of evaluating reproducibility a meta-analysis of each effect was performed. The forest plots, analyses, and data are available [here][6]. <br> Questions about the project can be directed to contact+rpcb@cos.io. [1]: https://doi.org/10.1038/nature09557 [2]: https://doi.org/10.7554/eLife.73430 [3]: https://elifesciences.org/articles/04363 [4]: https://osf.io/bnv9t/wiki/home [5]: https://osf.io/57p4y/wiki/home [6]: https://osf.io/2daz8/wiki/home