This project contains all information pertaining to the replication of experiments from “COT drives resistance to RAF inhibition through MAP kinase pathway reactivation” published in *Nature* in 2010 ([Johannessen et al., 2010][1]). Experimental work began although not all experiments were completed. The completed experiments were included in the summary paper "Experiments from unfinished Registered Reports in the Reproducibility Project: Cancer Biology" ([Errington et al., 2021][2]). <br> Also, explore the Registered Report and materials related to the Registered Report [here][3]. <br> **Data and Material Availability**: All other associated data, protocols, analysis scripts, and other digital materials are available within this OSF project. **Experiments replicated**: Reproduce and explore the figures, analyses, data, and methods generated in this replication attempt. - [PLX4720 growth inhibitory analysis in cells expressing elevated MAP3K8][4] - Replication of Figures 3D of Johannessen et al., 2010 - [MAPK pathway analysis after MAP3K8 inhibition in cells expressing elevated MAP3K8][5] - Replication of Figures 3I of Johannessen et al., 2010 - [Preliminary results of other experiments described in the Registered Report][6] - Attempt of replication of 3B, 3E, 4E, and 4F of Johannessen et al., 2010 <br> Questions about the project can be directed to contact+rpcb@cos.io. [1]: https://doi.org/10.1038/nature09627 [2]: https://doi.org/10.7554/eLife.73430 [3]: https://elifesciences.org/articles/11414 [4]: https://osf.io/bpnwx/wiki/home [5]: https://osf.io/kbtaq/wiki/home [6]: https://osf.io/seq9h/wiki/home