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<p>This is a project funded by the Betty and Gordon Moore Foundation as part of the Marine Microbial Initiative - Experimental Model Systems (MMI-EMS). We are building open source genetic tools for DNA fabrication based on the <a href="https://www.biorxiv.org/content/early/2018/01/18/247593" rel="nofollow">LOOP</a> assembly method for plants. All protocols, resources and data will be publicly released through OSF, <a href="http://protocols.io" rel="nofollow">protocols.io</a>, and GitHUB.</p> <p>"uLOOP" (from universal LOOP) is an open source, species-agnostic and simple DNA assembly tool being tested in yeast, diatoms, plants and bacteria.</p> <p>We have successfully built 4 versions of uLOOP (pSB from pSB4K5 vector from iGEM; pCO from pJT170 from Torella et al, 2014; pCA from pCambia; pAN from pAN3945 from Nielsen et al., 2016)</p> <p><a href="http://pAN_even.1.gb" rel="nofollow">pAN_even.1.gb</a> <a href="http://pAN_even.2.gb" rel="nofollow">pAN_even.2.gb</a> <a href="http://pAN_even.3.gb" rel="nofollow">pAN_even.3.gb</a> <a href="http://pAN_even.4.gb" rel="nofollow">pAN_even.4.gb</a> <a href="http://pAN_odd.1.gb" rel="nofollow">pAN_odd.1.gb</a> <a href="http://pAN_odd.2.gb" rel="nofollow">pAN_odd.2.gb</a> <a href="http://pAN_odd.3.gb" rel="nofollow">pAN_odd.3.gb</a> <a href="http://pAN_odd.4.gb" rel="nofollow">pAN_odd.4.gb</a></p> <p><a href="http://pCA_odd.1.gb" rel="nofollow">pCA_odd.1.gb</a> <a href="http://pCA_odd.2.gb" rel="nofollow">pCA_odd.2.gb</a> <a href="http://pCA_odd.3.gb" rel="nofollow">pCA_odd.3.gb</a> <a href="http://pCA_odd.4.gb" rel="nofollow">pCA_odd.4.gb</a> <a href="http://pCA_even.1.gb" rel="nofollow">pCA_even.1.gb</a> <a href="http://pCA_even.2.gb" rel="nofollow">pCA_even.2.gb</a> <a href="http://pCA_even.3.gb" rel="nofollow">pCA_even.3.gb</a> <a href="http://pCA_even.4.gb" rel="nofollow">pCA_even.4.gb</a></p> <p><a href="http://pCO_odd.1.gb" rel="nofollow">pCO_odd.1.gb</a> <a href="http://pCO_odd.2.gb" rel="nofollow">pCO_odd.2.gb</a> <a href="http://pCO_odd.3.gb" rel="nofollow">pCO_odd.3.gb</a> <a href="http://pCO_odd.4.gb" rel="nofollow">pCO_odd.4.gb</a> <a href="http://pCO_even.1.gb" rel="nofollow">pCO_even.1.gb</a> <a href="http://pCO_even.2.gb" rel="nofollow">pCO_even.2.gb</a> <a href="http://pCO_even.3.gb" rel="nofollow">pCO_even.3.gb</a> <a href="http://pCO_even.4.gb" rel="nofollow">pCO_even.4.gb</a></p> <p><a href="http://pSB_odd.1.gb" rel="nofollow">pSB_odd.1.gb</a> <a href="http://pSB_odd.2.gb" rel="nofollow">pSB_odd.2.gb</a> <a href="http://pSB_odd.3.gb" rel="nofollow">pSB_odd.3.gb</a> <a href="http://pSB_odd.4.gb" rel="nofollow">pSB_odd.4.gb</a> <a href="http://pSB_even.1.gb" rel="nofollow">pSB_even.1.gb</a> <a href="http://pSB_even.2.gb" rel="nofollow">pSB_even.2.gb</a> <a href="http://pSB_even.3.gb" rel="nofollow">pSB_even.3.gb</a> <a href="http://pSB_even.4.gb" rel="nofollow">pSB_even.4.gb</a></p> <p><strong>Comments, ideas and collaborations are welcome at all times!</strong>.</p> <p><strong>People:</strong></p> <p><strong>PUC Chile</strong>: Fernan Federici, Bernardo Pollak, Tamara Matute, Peter von Dassow, Constanza Lopez, Jorge Ibañez and Valentina Vargas, </p> <p><strong><a href="http://www.lcqb.upmc.fr/users/jaubert" rel="nofollow">UPMC</a> France:</strong> Marianne Jaubert and Angela Falciatore.</p> <p><strong><a href="http://www.jcvi.org/cms/home/" rel="nofollow">JCVI</a> US:</strong> Chris Dupont and Vincent Bielinski.</p> <p>“This research is funded by the Gordon and Betty Moore Foundation through Grant GBMF4981.01 to Fernán Federici and Peter von Dassow” </p>
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