<p>This is a project funded by the Betty and Gordon Moore Foundation as part of the Marine Microbial Initiative - Experimental Model Systems (MMI-EMS). We are building open source genetic tools for DNA fabrication based on the <a href="https://www.biorxiv.org/content/early/2018/01/18/247593" rel="nofollow">LOOP</a> assembly method for plants. All protocols, resources and data will be publicly released through OSF, <a href="http://protocols.io" rel="nofollow">protocols.io</a>, and GitHUB.</p>
<p>"uLOOP" (from universal LOOP) is an open source, species-agnostic and simple DNA assembly tool being tested in yeast, diatoms, plants and bacteria.</p>
<p>We have successfully built 4 versions of uLOOP (pSB from pSB4K5 vector from iGEM; pCO from pJT170 from Torella et al, 2014; pCA from pCambia; pAN from pAN3945 from Nielsen et al., 2016)</p>
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<p><strong>Comments, ideas and collaborations are welcome at all times!</strong>.</p>
<p><strong>People:</strong></p>
<p><strong>PUC Chile</strong>: Fernan Federici, Bernardo Pollak, Tamara Matute,
Peter von Dassow, Constanza Lopez, Jorge Ibañez and Valentina Vargas, </p>
<p><strong><a href="http://www.lcqb.upmc.fr/users/jaubert" rel="nofollow">UPMC</a> France:</strong>
Marianne Jaubert and Angela Falciatore.</p>
<p><strong><a href="http://www.jcvi.org/cms/home/" rel="nofollow">JCVI</a> US:</strong>
Chris Dupont and Vincent Bielinski.</p>
<p>“This research is funded by the Gordon and Betty Moore Foundation through Grant GBMF4981.01 to Fernán Federici and Peter von Dassow” </p>
<p>@<a href="https://mfr.osf.io/render?url=https://osf.io/cd28f/?action=download%26mode=render" rel="nofollow">osf</a>
ULoop decouples efficiency of assembly from efficiency of transformation, maintenaince and propagation in target organisms</p>
<p>@<a href="https://mfr.osf.io/render?url=https://osf.io/ju6dm/?action=download%26mode=render" rel="nofollow">osf</a>
uLoop allows the assembly of DNA of exponentially increasing size.</p>
<p>@<a href="https://mfr.osf.io/render?url=https://osf.io/8sywg/?action=download%26mode=render" rel="nofollow">osf</a></p>
