This is a project funded by the Betty and Gordon Moore Foundation as part of the Marine Microbial Initiative - Experimental Model Systems (MMI-EMS). We are building open source genetic tools for DNA fabrication based on the [LOOP][1] assembly method for plants. All protocols, resources and data will be publicly released through OSF, protocols.io, and GitHUB.
"uLOOP" (from universal LOOP) is an open source, species-agnostic and simple DNA assembly tool being tested in yeast, diatoms, plants and bacteria.
We have successfully built 4 versions of uLOOP (pSB from pSB4K5 vector from iGEM; pCO from pJT170 from Torella et al, 2014; pCA from pCambia; pAN from pAN3945 from Nielsen et al., 2016)
pAN_even.1.gb
pAN_even.2.gb
pAN_even.3.gb
pAN_even.4.gb
pAN_odd.1.gb
pAN_odd.2.gb
pAN_odd.3.gb
pAN_odd.4.gb
pCA_odd.1.gb
pCA_odd.2.gb
pCA_odd.3.gb
pCA_odd.4.gb
pCA_even.1.gb
pCA_even.2.gb
pCA_even.3.gb
pCA_even.4.gb
pCO_odd.1.gb
pCO_odd.2.gb
pCO_odd.3.gb
pCO_odd.4.gb
pCO_even.1.gb
pCO_even.2.gb
pCO_even.3.gb
pCO_even.4.gb
pSB_odd.1.gb
pSB_odd.2.gb
pSB_odd.3.gb
pSB_odd.4.gb
pSB_even.1.gb
pSB_even.2.gb
pSB_even.3.gb
pSB_even.4.gb
**Comments, ideas and collaborations are welcome at all times!**.
**People:**
**PUC Chile**: Fernan Federici, Bernardo Pollak, Tamara Matute,
Peter von Dassow, Constanza Lopez, Jorge Ibañez and Valentina Vargas,
**[UPMC][2] France:**
Marianne Jaubert and Angela Falciatore.
**[JCVI][3] US:**
Chris Dupont and Vincent Bielinski.
“This research is funded by the Gordon and Betty Moore Foundation through Grant GBMF4981.01 to Fernán Federici and Peter von Dassow”
@[osf](https://mfr.osf.io/render?url=https://osf.io/cd28f/?action=download%26mode=render)
ULoop decouples efficiency of assembly from efficiency of transformation, maintenaince and propagation in target organisms
@[osf](https://mfr.osf.io/render?url=https://osf.io/ju6dm/?action=download%26mode=render)
uLoop allows the assembly of DNA of exponentially increasing size.
@[osf](https://mfr.osf.io/render?url=https://osf.io/8sywg/?action=download%26mode=render)
[1]: https://www.biorxiv.org/content/early/2018/01/18/247593
[2]: http://www.lcqb.upmc.fr/users/jaubert
[3]: http://www.jcvi.org/cms/home/
[4]: https://osf.io/cd28f/
