Background information for reference
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**PBD files of interest**
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- Uba5 with ATP binded = 3H8V
- Uba5 with Ufm1 complex = 5iaa
- Uba5 with Ufm1 complex and AMP = 5L95
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## **ATP binding sites** ##
- G83
- D104
- K127
- N150
- N184
@[osf](4acjy)
Figure 1. Uba5 ATP binding sites G83 (red), D104 (green), K127 (blue), N150 (magenta), N184 (orange) and ATP (yellow) highlighted.
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## **Metal Binding Site and Active Site** ##
Metal
- C226
- C229
- Zn
Active
- Cys250
@[osf](k6q5s)
Figure 1. Uba5 metal binding sites C226 (red), C229 (blue) and Zn (purple) highlighted in upper right hand corner, active site Cys250 (cyan) highlighted in left hand corner, and ATP binding site highlighted in the center (various colors).
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## **Cys250 Active Site - Stabilizing residues** ##
@[osf](ejbds)
Figure 2. Cys250 active site with possible stabilizing side chains highlighted in various colors
