**Figure 1. Blood toxicity analysis.**
Female FVB mice bearing orthotopic MT1A2 breast tumors were randomized to receive IgG isotype control (IgG) (n=7) or anti-mouse CD47 (CD47) (n=7) antibodies. Mice bearing small or undetectable tumors were designated for baseline reading (NT) (n=6). For each parameter a one-way ANOVA was performed and the alpha level or p-value was adjusted using the Bonferroni correction. (**A**) White blood cells (WBC), one-way ANOVA; *F*(2,17) = 15.09, uncorrected *p* = .00017 with alpha level = .0033; (Bonferroni corrected *p* = .0026). (**B**) Neutrophils (NE), one-way ANOVA; *F*(2,17) = 1.02, uncorrected *p* = .381 with alpha level = .0033; (Bonferroni corrected *p* > .99). (**C**) Lymphocytes (LY), one-way ANOVA; *F*(2,17) = 20.84, uncorrected *p* = 2.67x10<sup>-5</sup> with alpha level = .0033; (Bonferroni corrected *p* = .00040). (**D**) Monocytes (MO), Welch's one-way ANOVA; *F*(2,8.52) = 9.98, uncorrected *p* = .0058 with alpha level = .0033; (Bonferroni corrected *p* = .0877). (**E**) Eosinophils (EO), one-way ANOVA; *F*(2,17) = 0.06, uncorrected *p* = .942 with alpha level = .0033; (Bonferroni corrected *p* > .99). (**F**) Basophils (BA), one-way ANOVA; *F*(2,17) = 4.20, uncorrected *p* = .0330 with alpha level = .0033; (Bonferroni corrected *p* = .495). (**G**) Red blood cells (RBC), one-way ANOVA; *F*(2,17) = 424.9, uncorrected *p* = 3x10<sup>-15</sup> with alpha level = .0033; (Bonferroni corrected *p* = 5xo<sup>-14</sup>). (**H**) Hemoglobin (Hb), one-way ANOVA; *F*(2,17) = 502.1, uncorrected *p* = 8xo<sup>-16</sup> with alpha level = .0033; (Bonferroni corrected *p* = 1x10<sup>-14</sup>). (**I**) Hematocrit (HCT), one-way ANOVA; *F*(2,17) = 283.0, uncorrected *p* = 9x10<sup>-14</sup> with alpha level = .0033; (Bonferroni corrected *p* = 1x10<sup>-12</sup>). (**J**) Mean corpuscular volume (MCV), one-way ANOVA; *F*(2,17) = 11.81, uncorrected *p* = .00061 with alpha level = .0033; (Bonferroni corrected *p* = .0091). (**K**) Mean corpuscular hemoglobin (MCH), one-way ANOVA; *F*(2,17) = 10.64, uncorrected *p* = .00101 with alpha level = .0033; (Bonferroni corrected *p* = .0151). (**L**) Mean corpuscular hemoglobin concentration (MCHC), one-way ANOVA; *F*(2,17) = 0.61, uncorrected *p* = .552 with alpha level = .0033; (Bonferroni corrected *p* > .99). (**M**) Red blood cell distribution width (RDW), Welch's one-way ANOVA; *F*(2,10.46) = 30.62, uncorrected *p* = 4.25x10<sup>-5</sup> with alpha level = .0033; (Bonferroni corrected *p* = .00064). (**N**) Platelets (PLT), one-way ANOVA; *F*(2,17) = 0.62, uncorrected *p* = .548 with alpha level = .0033; (Bonferroni corrected *p* > .99). (**O**) Mean platelet volume (MPV), one-way ANOVA; *F*(2,17) = 6.98, uncorrected *p* = .0061 with alpha level = .0033; (Bonferroni corrected *p* = .092).
Access [analysis script][1] for the statistical tests related to this figure. This script will call the [data][2] directly from the OSF.
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**Figure 2. Final tumor weights of immune competent hosts treated with control or CD47 targeted antibodies.**
At the end of the predefined study period (Day 31), tumors from mice bearing orthotopic MT1A2 breast tumors treated every other day with IgG isotype control (IgG) (n=7) or anti-mouse CD47 (anti-CD47) (n=6) antibodies were excised and weighed. Two-tailed Welch’s *t*-test between IgG and anti-CD47 treated tumors; *t*(9.66) = 1.796, *p* = .104.
Access [analysis script][3] for the statistical tests related to this figure. This script will call the [data][4] directly from the OSF.
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**Figure 2 - figure supplement 1. Tumor volumes of immune competent hosts treated with control or CD47 targeted antibodies.**
This is the same experiment as in Figure 2. Exploratory analysis on tumor weights excluding three tumors that regressed during the course of the study (indicated by asterisk): two-tailed Welch’s _t_-test between IgG and anti-CD47 treated tumors; _t_(7.94) = 0.745, _p_ = .478, Glass’ ∆ = -0.58, 95% CI [-1.88, 0.80].
Access [analysis script][3] for the statistical tests related to this figure. This script will call the [data][4] directly from the OSF.
[1]: https://osf.io/rq2nb/
[2]: https://osf.io/pcruk/
[3]: https://osf.io/y4q5r/
[4]: https://osf.io/qga8h/
