**Abstract** -------- Use of genetically-encoded optical probes to image calcium levels in neurons in vivo is used widely as a real-time measure of neuronal activity in the brain. Mesoscale calcium imaging through a cranial window provides a method of studying the interaction of circuit activity between cortical areas, but lacks access to subcortical regions. We have developed an optical and surgical preparation that preserves wide-field imaging of the cortical surface while also permitting access to subcortical networks. This was achieved by using an optical fiber implanted in the striatum, along with a bilateral widefield cranial window, enabling simultaneous mesoscale cortical imaging and subcortical fiber photometry recording of calcium signals in a transgenic animal expressing GCaMP. Subcortical signals were collected from dorsal regions of the striatum. We combined this approach with multiple sensory-motor tasks, including specific auditory and visual stimulation, and video monitoring of animal movements and pupillometry during head-fixed behaviors. We found high correlations between cortical and striatal activity in response to sensory stimulation or movement. Furthermore, spontaneous activity recordings revealed that specific (but not all) motifs of cortical activity are correlated with presynaptic activity recorded in the striatum, enabling us to select for corticostriatal activity motifs. This preparation can be used for the dual-color recording of most available green and red genetically-encoded sensors at cortical and subcortical levels with no significant crosstalk. We believe that this method can be utilized to reveal not only global patterns, but also cell-specific connectivities that provide insight into corticobasal ganglia circuit organization. Here, you can find the data (raw and preprocessed) recorded and analyzed for this project. **Data Structure** -------------- The data is provided as .mat MATLAB data files. The following variables are saved within each file. - **ctx (p x t)** The array containing the mesoscale cortical images (filtered dff). The ctx matrix is reshaped from the original 128x128xt, so that p is pixel (indexed as 1 to 128*128) and t is time with sampling frequency of 40 Hz. To visualize the cortical image at frame 'F', you can use the reshape function. e.g. `imagesc(reshape(ctx(:,F),128,128))` - **fiber (1 x t)** The Fiber Photometry axonal signal recorded from right dorsal striatum (filtered dff). The signal is filtered similar to the cortical signal, and is aligned and downsampled from 1017 Hz to 40 Hz. It should be same length (t) as the cortical signal. - **frame (X x Y x f)** The 3D array containing the video frames recorded from the behavior piCamera. The frames are double grayscale values. The video is at 10 fps and not upsampled to the brain signals. However, the start and end of the video is aligned with the start and end of the cortical and striatal recordings (f/10 = t/40). - **epoch (1 x n)** (Only in the sensory-evoked recordings) The array consists of the indices when a stimulus is delivered. The epoch(n) would refer to the index of the nth stimulus. For example the striatal signal for 1 second before to 2 seconds after the nth stimulus will be: `fiber(epoch(n)-40 : epoch(n)+80)` - **metadata (struct)** The metadata structure contains the animal ID, date of recording, sampling rates for each recording, and type of the experiment (vis-evoked, aud-evoked, spon).