## Registered report: Widespread potential for growth factor-driven resistance to anticancer kinase inhibitors <br> **Abstract:** The [Reproducibility Project: Cancer Biology][1] seeks to address growing concerns about reproducibility in scientific research by conducting replications of 50 papers in the field of cancer biology published between 2010 and 2012. This Registered Report describes the proposed replication plan of key experiments from ‘Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors’ by Wilson and colleagues, published in *Nature* in 2012 ([Wilson et al., 2012][2]). The experiments that will be replicated are those reported in Figure 2B and C. In these experiments, Wilson and colleagues show that sensitivity to receptor tyrosine kinase (RTK) inhibitors can be bypassed by various ligands through reactivation of downstream signaling pathways (Figure 2A; [Wilson et al., 2012][3]), and that blocking the receptors for these bypassing ligands abrogates their ability to block sensitivity to the original RTK inhibitor (Figure 2C; [Wilson et al., 2012][4]). The Reproducibility Project: Cancer Biology is a collaboration between the [Center for Open Science][5] and [Science Exchange][6], and the results of the replications will be published by [*eLife*][7]. ---------- ### Contents This component contains additional materials relating to the Registered Report for Study 46: Wilson et al. 2012, Nature. The Registered Report can be found [here][8]. ---------- **Registered Report Initial Draft**: - This document represents the first draft of the selected experimental protocols. It contains the information that the Core team, with help from scientist volunteers, was able to glean from the original publication and its supplemental materials before the original authors were contacted for their input. - <a href="https://osf.io/6qcrk/">Study 46 Registered Report Initial Draft</a> **Data Estimation**: - When original data was unable to be obtained, or original qualitative data was quantified (for example, Western blots), the estimated data is contained here. Examples include densitometric quantification of Western blot bands, or estimations of means and variances from published graphs. - <a href="https://osf.io/n7gv8/">Study 46 Figure 2C: Western Blot quantification</a> [1]: https://osf.io/e81xl/wiki/home/ [2]: https://doi.org/10.1038/nature11249 [3]: https://doi.org/10.1038/nature11249 [4]: https://doi.org/10.1038/nature11249 [5]: http://centerforopenscience.org/ [6]: https://www.scienceexchange.com/ [7]: https://elifesciences.org/ [8]: http://elifesciences.org/content/3/e04037