--- The role of osmorespiratory compromise in metabolism and hypoxia tolerance of a purportedly oxyconforming teleost --- <br> [](https://creativecommons.org/licenses/by/4.0/) <br> > This work is licensed under a [Creative Commons Attribution 4.0 International License](https://creativecommons.org/licenses/by/4.0/). You are free to reuse, redistribute, and adapt the material, provided appropriate credit is given. <br> --- <br> ## 📄 Project Summary <br> - **Title**: The role of osmorespiratory compromise in metabolism and hypoxia tolerance of a purportedly oxyconforming teleost <br> - **Authors**: Timothy D. Clark[1], Luis L. Kuchenmüller[1], Elizabeth C. Hoots[1], Maryane Gradito[1], and Jake M. Martin[1,2] <br> - **Institutions**: [1] School of Life and Environmental Sciences, Deakin University, Geelong, VIC, Australia, and [2] Department of Wildlife, Fish and Environmental Studies, Swedish University of Agricultural Sciences, Umeå, Sweden <br> This project tests: 1. Whether *Galaxias maculatus* exhibits oxyregulation 2. Whether isosmotic conditions reduce resting oxygen uptake and improve hypoxia tolerance <br> The study compares SMR, RMR, and estimated o2crit values across salinity treatments (0 ppt vs 9 ppt), using data collected from intermittent-flow respirometry and analysed in R (v4.2.3). 📍 [Browse the HTML output](https://jakemartinresearch.github.io/gmac-mo2-24/) <br> --- <br> ## 📁 Repository Structure <br> ``` 📁 gmac-mo2-24/ ├── 📂 input-data/ (Raw and cleaned input data) ├── 📂 mod-data/ (Intermediate summaries for modelling) ├── 📂 output-fig/ (Final figures used in the manuscript) ├── 📂 output-mod/ (Model outputs (.rds files)) ├── 📄 gmac-labchart-24.Rmd (Main analysis and figure script) └── 📄 supplementary-file-1.html (Supplementary file) ``` <br> --- <br> ## 📦 R Dependencies <br> This project uses the following key R packages: **🔹 Data Visualisation** - `ggthemes` - `bayesplot` - `gt` - `gtsummary` - `plotly` - `qqplotr` **🔹 Tidy Data & Wrangling** - `tidyverse` - `janitor` - `readxl` - `broom.mixed` - `data.table` - `hms` - `devtools` - `mclust` **🔹 Modelling & Statistical Analysis** - `brms` - `rstan` - `marginaleffects` - `performance` - `emmeans` - `tidybayes` - `respirometry` - `future` These are installed and loaded in the `.Rmd` using `pacman::p_load(...)`. <br> --- <br> ## 🔍 Key Methods <br> - **SMR estimation**: Using a custom version of the `calcSMR` method (Chabot et al., 2016) - **Incremental regression**: Polynomial Bayesian models to describe ṀO₂–DO relationships (Urbina et al., 2012) - **o2crit detection**: Rule-based detection method with visual validation (Claireaux & Chabot, 2016) - **Model comparison**: Leave-one-out cross-validation (LOO-CV) for evaluating best-fit models <br> --- ## 📊 Outputs <br> - HTML-rendered results and figures are available at: 📍 [https://jakemartinresearch.github.io/gmac-mo2-24/](https://jakemartinresearch.github.io/gmac-mo2-24/) - Bayesian model outputs are saved as `.rds` files and stored in the `/output-mod/` folder. --- <br> ## 📘 Citation <br> 1. **Licenses/restrictions placed on the data:** CC-BY 4.0 <br> 2. **Link to the associated publication:** <br> 🚧 ***To be added*** 🚧 <br> 3. **Recommended citation for this data:** <br> Clark, T. D., Kuchenmüller, L. L., Hoots, E. C., Gradito, M., & Martin, J. M. (2025, May 28). The role of osmorespiratory compromise in metabolism and hypoxia tolerance of a purportedly oxyconforming teleost. https://doi.org/10.17605/OSF.IO/GFXCA <br> --- <br> ## 📩 Contact <br> **Jake M. Martin** <br> 📧 **Email:** [jake.martin@deakin.edu.au](mailto:jake.martin@deakin.edu.au) 📧 **Alt Email:** [jake.martin.research@gmail.com](mailto:jake.martin.research@gmail.com) 🌐 **Web:** [jakemartin.org](https://jakemartin.org/) 🧪 **ORCID**: [0000-0001-9544-9094](https://orcid.org/0000-0001-9544-9094) ---