## Registered report: Kinase-dead BRAF and oncogenic RAS cooperate to drive tumor progression through CRAF <br> **Abstract:** The [Reproducibility Project: Cancer Biology][1] seeks to address growing concerns about reproducibility in scientific research by conducting replications of selected experiments from a number of high-profile papers in the field of cancer biology. The papers, which were published between 2010 and 2012, were selected on the basis of [citations and Altmetric scores][2] ([Errington et al., 2014][3]). This Registered Report describes the proposed replication plan of key experiments from "Kinase-dead BRAF and oncogenic RAS cooperate to drive tumor progression through CRAF" by Heidorn and colleagues, published in *Cell* in 2010 ([Heidorn et al., 2010][4]). The experiments to be replicated are those reported in Figures 1A, 1B, 3A, 3B, and 4D. Heidorn and colleagues report that paradoxical activation of the RAF-RAS-MEK-ERK pathway by BRAF inhibitors when applied to BRAF^WT cells is a result of BRAF/CRAF heterodimer formation upon inactivation of BRAF kinase activity, and occurs only in the context of active RAS. The Reproducibility Project: Cancer Biology is a collaboration between the [Center for Open Science][5] and [Science Exchange][6], and the results of the replications will be published by [*eLife*][7]. ---------- ### Contents This component contains additional materials relating to the Registered Report for Study 7: Heidorn et al., 2010, *Cell*. The Registered Report can be found [here][15]. ---------- **Registered Report Initial Draft**: - This document represents the first draft of the selected experimental protocols. It contains the information that the Core team, with help from scientist volunteers, was able to glean from the original publication and its supplemental materials before the original authors were contacted for their input. - [Study 7 Registered Report Initial Draft][16] **Power Calculations**: - These documents contain details about how power calculations were performed, and include spreadsheets documenting statistical tests as well as any PRISM files, R scripts or additional materials used. - [Study 7 Power Calculations][17] - [Study 7 Protocol 1 Figure 1A 2-way ANOVA.R][18] - [Study 7 Protocol 2 Figure 1B 1-way ANOVA.R][19] - [Study 7 Protocol 3 Figure 3A 2-way ANOVA.R][20] - [Study 7 Protocol 4 Figure 3B 2-way ANOVA.R][21] - [Study 7 Protocol 5 Figure 4D Welch's t-tests.R][22] [1]: https://osf.io/e81xl/wiki/home/ [2]: https://osf.io/e81xl/wiki/studies/ [3]: https://doi.org/10.7554/eLife.04333 [4]: https://doi.org/10.1016/j.cell.2009.12.040 [5]: http://centerforopenscience.org/ [6]: https://www.scienceexchange.com/ [7]: https://elifesciences.org/ [8]: http://elifesciences.org/collections/reproducibility-project-cancer-biology [9]: https://osf.io/xq93y/ [10]: https://osf.io/y83jb/ [11]: https://osf.io/wezf4/ [12]: https://osf.io/dg39c/ [13]: https://osf.io/sb2dm/ [14]: https://osf.io/p4qhs/ [15]: https://elifesciences.org/articles/11999 [16]: https://osf.io/xq93y/ [17]: https://osf.io/y83jb/ [18]: https://osf.io/wezf4/ [19]: https://osf.io/dg39c/ [20]: https://osf.io/sb2dm/ [21]: https://osf.io/p4qhs/ [22]: https://osf.io/537e2/