Contains for every promoter a tab delimited file with all barcodes used in this experiment. Barcodes were uniquely mapped to a single position in the genome.
Promoters were classified according to Leemans *et al.* in one of the following classes:
|class | description |
|----------|-------------|
|iLAD | not associated with the lamina |
|escaper | promoter is associated with the lamina, but endogenously is not repressed by this association
|repressed | promoter is associated with the lamina and actively repressed by it's association
7 promoters used in this experiment:
|Symbol | ID ([gencode v27][1]) | class |
|---------|-----------------------|-----------|
|ADAMTS1 | ENSG00000154734.14 | repressed |
|ARHGEF9 | ENSG00000131089.15 | repressed |
|BRINP1 | ENSG00000078725.12 | repressed |
|MED30 | ENSG00000164758.7 | escaper |
|PGK | ENSG00000102144.13 | iLAD |
|TMEM106B | ENSG00000106460.18 | escaper |
|ZNF300 | ENSG00000145908.12 | escaper |
For each promoter 2 cell pools were established, integrations were mapped and expression was measured. Each tab-delimeted file contains the following columns:
|name | description |
|------------|-------------|
|seqname | sequence name of the integration site (hg38) |
|strand | orientation of integration site (hg38) |
|position | position of the site of integration (hg38)
|barcode | 16bp barcode of integration |
|pool | which of the 2 cell-pools per promoter the barcode was found |
|expression | normalized expression : (expression count + 1) / abundance |
|LMNB1_DamID | log2(LmnB1-Dam/Dam) of 10kb window centered on integration site |
|lad | LAD state at site of integration, called by HMM on DamID data |
[1]: https://www.gencodegenes.org/human/release_27.html