Target of replication: *A priori* experimental protocols, materials, and confirmatory analysis plans were peer reviewed, [pre-registered][1], and published by [*eLife*][2]. This project includes all primary data, analysis scripts, and QC data for the replication of the experiment reported in Figure 2B of [Sugahara et al. 2010][3]. This experiment was performed to assess the impact on DOX accumulation in various tissue of mice treated with 10 mg/kg DOX + PBS or 10 mg/kg DOX + 4 µmol/kg for 1 hr. <br> ### Contents [Figures component][4] contains a visualization of replication results. These figures are the same as can be found in the Replication Study and include Figure 1 displaying DOX accumulation in mouse tissues. [Statistical Analyses component][5] contains R scripts that were used for the analyses. [Data Component][6] contains data generated from the experimental work and files used in the analysis and generation of figures, which are in .csv format. [Methods component][7] contains methodology details for this replication attempt which were described in the [Registered Report][8]. [Cell line quality control data][9] includes results confirming the cell lines were free of mycoplasma contamination and common mouse pathogens as well as STR DNA profiling of the cell lines used. [iRGD peptide component][10] contains the methods and quality control data generated during the synthesis. [1]: https://osf.io/9hr2d/ [2]: http://elifesciences.org/content/4/e06959v1 [3]: http://science.sciencemag.org/content/328/5981/1031 [4]: https://osf.io/zmbrs/wiki/home [5]: https://osf.io/u2nwm/wiki/home [6]: https://osf.io/678ph/wiki/home [7]: https://osf.io/bkhnp/wiki/home [8]: http://elifesciences.org/content/4/e06959v1 [9]: https://osf.io/en6ru/wiki/home [10]: https://osf.io/p73gy/wiki/home/