Complete documentation on how to run SuperCRUNCH is available at: https://github.com/dportik/SuperCRUNCH/wiki
The following generalized commands were used to run the analysis. Please note that the `/Path/To/` should be changed to the actual file locations.
This analysis simply removed markers with fewer than 75 sequences.
```
python Fasta_Filter_by_Min_Seqs.py -i /Path/To/6-Minimum-Sequences/input --min_seqs 75 -o /Path/To/6-Minimum-Sequences/output > Fasta_Filter_by_Min_Seqs.log
```
It produced the following results:
```
Found 119 total fasta files.
Processing...
113 fasta files passed the minimum sequence filter (>=75 seqs).
6 fasta files failed the minimum sequence filter (>=75 seqs).
Fasta files with >= 75 records have been copied to: /Users/dportik/Documents/Projects/FROGS2/7-Min-Seqs/Filtered-Fasta-Files
Fasta files failing filter:
MC1R_extracted_oneseq.fasta
MYH_extracted_oneseq.fasta
NPCL_B3GALT1_extracted_oneseq.fasta
NPCL_FUT9_extracted_oneseq.fasta
NPCL_ZBED4_extracted_oneseq.fasta
ZFX_extracted_oneseq.fasta
```