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WIKI NAVIGATION
*[Introduction to Banyuls Experiment][1]*
*[Contextual data][2]*
*[Sequencing data][3]*
*[Taxonomic and functional annotation][4]*
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Taxonomic and functional annotation
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The EBI Metagenomics Analysis tool for EMOSE data is accessible here: [https://wwwdev.ebi.ac.uk/metagenomics/projects/ERP090011][5]
The tool offers taxonomic and functional assignation for all experiments (i.e. runs) from the following five sequencing & library construction protocols:
1) **(Emose_metagenomics_WGS)** Shotgun DNA sequencing;
2) **(Emose_metaBarcoding_18S)** Amplicon sequencing after 18S amplification by PCR using 1391F/EukB primer set. Library were constructed according to Illumina Library protocol without any sizing;
3) **(Emose_metaBarcoding_16S_noSizing)** Amplicon sequencing after 16S amplification by PCR using 515F/926R primer set. Library were constructed according to Illumina Library protocol without any sizing;
4) **(Emose_metaBarcoding_16S_sizing_450-650bp)** Amplicon sequencing after 16S amplification by PCR using 515F/926R primer set. Library were constructed according to Illumina Library protocol with a sizing step selecting the 450-650 bp fragments; and
5) **(Emose_metaBarcoding_16S_sizing_650-850bp)** Amplicon sequencing after 16S amplification by PCR using 515F/926R primer set. Library were constructed according to Illumina Library protocol with a sizing step selecting the 650-850 bp fragments.
The analysis results can be sorted & filtered based on the sample name and experiment type, which distinguishes between Metagenomics and Amplicon sequencing and library construction protocols. Unfortunately, the tool cannot distinguish the four Amplicon protocols used in EMOSE.
**Alternatively, you can explore/filter/select/access the analysis results using the worksheet** ***EMOSE_SAMPLES_EBI-Analysis-LINKS*** in the Google Spreadsheet [EMOSE (2017) - Banyuls Experiment - Data - Sample_Registry_20170815.xlsx][6]
[1]: https://osf.io/jv7az/wiki/home
[2]: https://osf.io/ane6f/wiki/home/
[3]: https://osf.io/yhbxj/wiki/home/
[4]: https://osf.io/bkh8f/wiki/home/
[5]: https://wwwdev.ebi.ac.uk/metagenomics/projects/ERP090011
[6]: https://docs.google.com/spreadsheets/d/1Rfx9Es34NbhdH9FU5blG3xXPAKnvXMtPSdvA8r4vOm0/edit?usp=sharing
