## Replication Study: The CD47-signal regulatory protein alpha (SIRPa) interaction is a therapeutic target for human solid tumors
In 2015, as part of the [Reproducibility Project: Cancer Biology], we published a Registered Report ([Chroscinski et al., 2015]), that described how we intended to replicate selected experiments from the paper “The CD47-signal regulatory protein alpha (SIRPa) interaction is a therapeutic target for human solid tumors“ ([Willingham et al., 2012]). Here we report the results of those experiments. We found that treatment of immune competent mice bearing orthotopic breast tumors with anti-mouse CD47 antibodies resulted in short-term anemia compared to controls, consistent with the previously described function of CD47 in normal phagocytosis of aging red blood cells and results reported in the original study (Table S4; [Willingham et al., 2012]). The weight of tumors after 30 days administration of anti-CD47 antibodies or IgG isotype control were not found to be statistically different, whereas the original study reported inhibition of tumor growth with anti-CD47 treatment (Figure 6A,B; [Willingham et al., 2012]). Additionally, the excised tumors were scored for inflammatory cell infiltrates. Both IgG and anti-CD47 treated tumors resulted in minimal to moderate lymphocytic infiltrate, while neutrophilic infiltration was slightly increased in anti-CD47 treated tumors, while the original study observed sparse lymphocytic infiltrate in IgG-treated tumors and increased inflammatory cell infiltrates in anti-CD47 treated tumors (Figure 6C; [Willingham et al., 2012]). Finally, we report a meta-analysis of the result.
**Reports:** Read the [Replication Study], or view the [preprint versions].
To reproduce the Replication Study manuscript text run this in [R Studio] (note: downloads [R markdown file] directly from osf.io):
GET("https://osf.io/bsefc/?action=download", write_disk("Replication_Study_39.Rmd", overwrite = T))
render("Replication_Study_39.Rmd", output_format = "word_document")
Also, explore the [Registered Report] and materials related to the Registered Report [here].
**Experiments replicated:** Reproduce and explore the figures, analyses, data, and methods generated in this replication attempt.
- [Engraftment of mouse breast cancer cells and treatment with CD47 targeted antibodies]
- Replication of Figures 6A-C and Table S4 in Willingham et al., 2012.
**Meta-analyses:** As a measure of evaluating reproducibility a meta-analysis of each effect was performed. The forest plots, analyses, and data are available [here].
Questions about the project can be directed to [email@example.com].