Computer-simulated virtual environments (VE) allow subjects to perform simulations of real-life tasks usually impractical within the confines of an fMRI scanner. In the first known test of the correspondence of behavior between real life and a VE (Appleby et al., 2008) played within an fMRI scanner, 120 gay men aged 18-30 (including 40 methamphetamine users) made decisions in the VE predicting real-life behavior. Subjects who made sexually risky decisions during gameplay reported having more unsafe sex in the previous 90 days (N=25, Mdn=5) than other subjects (N=95, Mdn=2), W=825.5, p=.017, r=.27. In-game sex positions predicted real-life sex positions (ρ[43]=0.764, p<0.001). In-game alcohol consumption predicted drinks per week (ρ[120]=0.219, p=0.016) and time since last drink (ρ[120]=-0.209, p=0.023). Results suggest VE could be used in fMRI experiments to enhance ecological validity. Ongoing work examines relationships between in-game conversational behaviors and personality measures.