## Registered report: Interactions between cancer stem cells and their niche govern metastatic colonization <br> **Abstract:** The [Reproducibility Project: Cancer Biology][1] seeks to address growing concerns about reproducibility in scientific research by replicating selected results from a substantial number of high-profile papers in the field of cancer biology published between 2010 and 2012. This Registered report describes the proposed replication plan of key experiments from ‘Interactions between cancer stem cells and their niche govern metastatic colonization’ by Malanchi and colleagues, published in *Nature* in 2012 ([Malanchi et al., 2012][2]). The key experiments that will be replicated are those reported in Figures 2H, 3A, 3B, and S13. In these experiments, Malanchi and colleagues analyze messenger RNA levels of periostin (POSTN) in pulmonary fibroblasts, endothelial cells, and immune cells isolated from mice with micrometastases to determine which cell type is producing POSTN in the metastatic niche (Figure 2H; [Malanchi et al., 2012][3]). Additionally, they examine MMTV-PyMT control or POSTN null mice to test the effect of POSTN on primary tumor growth and metastasis (Figures 3A, 3B, and S13; [Malanchi et al., 2012][4]). The Reproducibility Project: Cancer Biology is a collaboration between the [Center for Open Science][5] and [Science Exchange][6], and the results of the replications will be published in [*eLife*][7]. ---------- ### Contents This component contains additional materials relating to the Registered Report for Study 43: Malanchi et al. 2012, Nature. The Registered Report can be found [here][8]. ---------- **Registered Report Initial Draft**: - This document represents the first draft of the selected experimental protocols. It contains the information that the Core team, with help from scientist volunteers, was able to glean from the original publication and its supplemental materials before the original authors were contacted for their input. - [Study 43 Registered Report Initial Draft][9] **Power Calculations**: - These documents contain details about how power calculations were performed, and include spreadsheets documenting statistical tests as well as any PRISM files, R scripts or additional materials used. - [Study 43 Power calculations][10] - [Protocol 43 data estimation][11] - [Study 43 Protocol 1 normality homoscedasiticity tests.R][12] - [Study 43 Protocol 1 regression model.R][13] - [Study 43 Protocol 2 1-way ANOVA.R][14] [1]: https://osf.io/e81xl/wiki/home/ [2]: https://doi.org/10.1038/nature10694 [3]: https://doi.org/10.1038/nature10694 [4]: https://doi.org/10.1038/nature10694 [5]: http://centerforopenscience.org/ [6]: https://www.scienceexchange.com/ [7]: https://elifesciences.org/ [8]: http://elifesciences.org/content/4/e06938 [9]: https://osf.io/tcauf/ [10]: https://osf.io/38p9w/ [11]: https://osf.io/bsdzq/ [12]: https://osf.io/qjmvx/ [13]: https://osf.io/cbjut/ [14]: https://osf.io/8mwen/