Brainstorming - Questions to address ------------------------------------ The end product of EMOSE 2017 will be a methods paper that recommends best sampling and sequencing practices for marine plankton genomic observatories. The first thing to remember is that all EMOSE samples were collected in the same environment in order to compare different methods. Replicates are available for each method used. A first set of analyses should test the variability of results among replicates and between the two sampling days. We should always be cautious when combining data from the two sampling days. The different filtration methods vary in terms of the filtration volumes (1, 2.5, 10, 100, 500 &nd 1000L), devices (sterivex vs. membrane), and size-fractionation technique (none, serial and with a filtrate collection step). The following figure illustrates some questions that we could address with this data. The different sequencing and library construction protocols used by Genoscope will help address the different questions raised in that figure, but should also drive question that compare sequencing strategies, e.g. comparing the three 16S protocols. The use of MOCK communities provided by Jed Fuhrman's lab should also help test our confidence in the sequencing results. **ADD/COMMENT questions that we should address during the Hackathon here**: [https://docs.google.com/document/d/1FD1-rNiw8i1rlcgX2Orp1z7pI191Lm-krQYq8_DG0r8/edit?usp=sharing][1] ![][2] [1]: https://docs.google.com/document/d/1FD1-rNiw8i1rlcgX2Orp1z7pI191Lm-krQYq8_DG0r8/edit?usp=sharing [2]: https://osf.io/zjcsh/?action=download&direct&mode=render