Paired reaches at seven locations within the Kiamichi and Little rivers in southeastern Oklahoma were sampled twice a year in 2015 and 2016 to understand the influence of mussel beds on fish and macroinvertebrate distribution and biomass, and how mussel and fish aggregations influence nutrient cycling and resource abundance and dynamics. Much of these data are published in Hopper et al. (2018) Oecologia and the data are archived here. The macroinvertebrate data are in a manuscript that is in review and will be archived here upon publication.