Resolving the Three-Dimensional Rotational and Translational Dynamics of Single Molecules Using Radially and Azimuthally Polarized Fluorescence

doi:10.17605/OSF.IO/64BFV

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@[OSF](ydz65) Raw radially and azimuthally polarized (raPol) images from Nile red tracking experiments with 5 mW TIRF excitation and (a-c) 100 ms and (d) 10 ms exposure times. Images of the supported lipid bilayer (a) before the MβCD treatment, (b) after a 40 μM MβCD treatment, and (c,d) after an 80 μM MβCD treatment. Red and blue represent the radially and azimuthally polarized imaging channels. Scale bar: 2 μm; colorbar: photons.

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