Using [functional magnetic resonance imaging (fMRI)][1], we studied links between manual praxis ([planned and executed tool use pantomimes][2]), language (in [subvocal word generation task][3]) and handedness in 125 participants, including righthanders (N=52), ambidextrous individuals (mixedhanders; N=31), and lefthanders (N=42). The tested sample included 64 females and 61 males, MA = 22.6 years, and SD of ± 3.1 years (range = 20, min. 19 and max. 39) at the time of fMRI testing. All were healthy volunteers, native speakers of Polish, typically students of, or graduates from, [Poznan][4] universities. All neuroimaging data were acquired with the use of a 3-Tesla Trio MRI scanner ([Siemens Medical Solutions][5]), enabling echo planar imaging (EPI), with a 32-channel PA head coil, for Radio Frequency transmission/signal reception. The echoplanar BOLD images were acquired using T2*-weighted segmented gradient-echo imaging sequence with the following parameters: field of view (FOV) = 196 mm; 64x64 matrix; in-plane resolution = 3.06x3.06 mm; flip angle (FA) = 90°; time to echo (TE) = 30 ms; time to repetition (TR) = 2,000 ms. Each volume contained 35 contiguous axial slices of 3.1 mm thickness. The first four volumes in each scan series were always discarded. Standard, anatomical scans were acquired in both sessions, with the use of a 3D T1-weighted magnetization prepared rapid gradient echo (MP-RAGE) pulse sequence, with the following parameters: TE/TR = 3.32/2,530 ms; inversion time (TI) = 1,200 ms; FA = 7°; 176 contiguous axial slices; in-plane resolution = 1.0×1.0 mm; slice thickness = 1.0 mm; voxel matrix size = 256x176; FOV = 256 mm. Finally, fast spin echo T2-weighted anatomical images were also collected, with the following parameters: TR = 3,200 ms; TE = 402 ms; FA = 120°; 512×512 voxel matrix size; FOV = 256mm; 176 contiguous sagittal slices; in-plane resolution = 0.5×0.5; slice thickness = 1mm. The obtained DICOM images were converted to NIfTI ([FSL][6]) format using [MRI-Convert software][7]. [Laterality][8] indices were calculated both in two critical [cytoarchitectonic maps][9], and 180 [multi-modal parcellations][10] of the human [cerebral cortex][11], using voxel count and signal intensity, and the most relevant regions of interest and their networks were further analyzed. For more details, see our [Cortex paper][12]. [1]: https://en.wikipedia.org/wiki/Functional_magnetic_resonance_imaging [2]: https://actaneuropsychologica.com/api/files/view/1163690.pdf [3]: https://actaneuropsychologica.com/api/files/view/1163690.pdf [4]: https://en.wikipedia.org/wiki/Pozna%C5%84 [5]: https://www.siemens-healthineers.com [6]: https://fsl.fmrib.ox.ac.uk/fsl/fslwiki [7]: http://lcni.uoregon.edu/~jolinda/MRIConvert/ [8]: https://en.wikipedia.org/wiki/Laterality [9]: https://en.wikipedia.org/wiki/Cytoarchitecture [10]: https://www.nature.com/articles/nature18933 [11]: https://en.wikipedia.org/wiki/Cerebral_cortex [12]: https://www.sciencedirect.com/science/article/pii/S0010945221001337