**Figure 2. Impact of *pks* island deletion on mouse survival.** Contains both a Kaplan-Meier plot of overall survival starting at time of mono-association until 18 weeks after the last AOM injection, which is the experimental timing used in this replication, as well as a Kaplan-Meier plot of overall survival starting at time of mono-association until 18 weeks after mono-association, which is the experimental timing used in the original study (Arthur et al., 2012). Access the [full size image][1] and [R script][2] used to generate this figure. **Figure 2 - figure supplement 1. Impact of *pks* island deletion on bacterial growth *in vivo.*** Contains the quantified colony forming units (CFU/ml) collected at sacrifice for Il10-/- germ-free mice treated with AOM and mono-associated with either *E. coli* NC101 *E. coli* NC101*Δpks*. Access the [full size image][3] and [R script][4] used to generate this figure. **Figure 2 - figure supplement 2. Histopathology of mouse tissues.** Includes representative images from the same experiment as in Figure 2. Tissues were fixed, sectioned, and stained with hematoxylin and eosin (H&E) Access the [full size image][5] used to generate this figure as well as the representative images used in figure supplements [here][6]. **Figure 3. Impact of pks island deletion on colonic inflammation and tumorigenesis.** Contains dot plots where each symbol represents data from one mouse with medians reported as crossbars. Includes, macroscopic tumor number where individual tumors were detectable, histological inflammation scores, histological invasion scores, and histological neoplasia scores. Access the [full size image][7] used to generate this figure as well as the representative images used in figure supplements [here][8]. **Figure 3 - figure supplement 1. Gross appearance of mouse colon.** Includes representative images of both a colon and Mucosal surface of the distal colon from the same tissue. Access the [full size image][9] used to generate this figure as well as the analysis script used to generate this figure [here][6]. **Figure 3 - figure supplement 2. Illustrative photomicrographs of scoring system used to semi-quantify colon inflammation.** Includes representative images of (**A**) a normal colon from a germ-free mouse for comparison, (**B**) a colon from a mouse mono-associated with NC101 and euthanized 27 weeks after bacterial inoculation, (**C**) a colon from a mouse mono-associated with NC101 and euthanized 27 weeks after bacterial inoculation, (**D**) a colon from a mouse mono-associated with NC101Δpks and euthanized 27 weeks after bacterial inoculation and (**E**) a colon from a mouse mono-associated with NC101 and euthanized 25 weeks after bacterial inoculation. Access the [full size image][10] used to generate this figure as well as the representative images used in figure supplements [here][6]. **Figure 3 - figure supplement 3. Illustrative photomicrographs of scoring system used to quantify proliferative, dysplastic, and neoplastic lesions.** Includes representative images of (**A**) Colon from a mouse mono-associated with NC101 and euthanized 23 weeks after bacterial inoculation, (**B**) Colon from a mouse mono-associated with NC101 and euthanized 25 weeks after bacterial inoculation, and (**C**) Invasive colon adenocarcinoma from the same mouse illustrated in A, mono-associated with NC101 and euthanized 23 weeks after bacterial inoculation. Access the [full size image][11] used to generate this figure as well as the representative images used in figure supplements [here][6]. [1]: https://osf.io/7wsg2/ [2]: https://osf.io/ezhyk/ [3]: https://osf.io/u86re/ [4]: https://osf.io/u7waz/ [5]: https://osf.io/ufpcg/ [6]: https://osf.io/fu4rn/ [7]: https://osf.io/qta26/ [8]: https://osf.io/nzypt/ [9]: https://osf.io/zx92u/ [10]: https://osf.io/avsnh/ [11]: https://osf.io/vqhbg/