Here we make available the scenes and software to reproduce the paradigm used in the article “A 5-minute paradigm to evoke robust emotional reactivity in neuroimaging studies” article. In the original experiment, the scenes were presented using the Psychophysics Toolbox (Brainard, 1997) which runs in Matlab (Mathworks, Inc.). To enable researchers to avoid a dependence on a Matlab license, the presentation paradigm was rewritten using open-source Psychopy software (Peirce et al., 2019). The code was created with the “builder” graphic user interface such that it can be simple to use and adjust for individual research applications. The code includes a generic trigger wait loop to enable synchronization with the first functional image acquisition, which will need to be set appropriately for each scanner and chosen image repeat time (the default is a 2 second TR). In addition, the scene presentation order is randomized for each run, with a requirement that a scene depicting one of the 5 categories of emotional and neutral contents would not be shown in succession (erotica, joy, neutral, threat, and mutilation). This process occurs before the experiment is fully loaded. There is also a means of tracking and correcting the slight time variation (~ 1-2 ms) that occurs as each scene is loaded into video memory. This variation is corrected in the following inter trial interval, such that each trial has the same duration, and the total presentation series ends on time. The scene presentation order and onset / offset times are recorded in a log file for each run. The scene series begins with a 2 s checkerboard acclimation image, followed by the 20 experimental scenes, presented for 2 s each, separated by interstimulus intervals of 10 or 12 s. The total acquisition time was 4 minutes and 42 seconds, including 5 dummy acquisitions (10 s) prior to the first trial to allow MR signal stabilization. Brainard, D. H., & Vision, S. (1997). The psychophysics toolbox. Spatial vision, 10(4), 433-436. Peirce, J., Gray, J. R., Simpson, S., MacAskill, M., Höchenberger, R., Sogo, H., Kastman, E., & Lindeløv, J. K. (2019). PsychoPy2: Experiments in behavior made easy. Behavior research methods, 51(1), 195-203. https://doi.org/10.3758/s13428-018-01193-y