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Target of replication: *A priori* experimental protocols, materials, and confirmatory analysis plans were peer reviewed, [pre-registered][1], and published by [*eLife*][2]. This component includes all primary data, analysis scripts, and QC data for the replication of the experiment reported in Figure S6A of [Berger et al. 2012][3]. This experiment was performed to assess the protein expression of PREX2 in NRAS<sup>G12D</sup> melanocytes which stably expressed GFP, PREX2<sup>WT</sup>, PREX2<sup>G844D</sup>, or PREX2<sup>Q1430*</sup>. Endogenous PREX2 was probed as well as the exogenously expressed variants. PREX2 expression was compared between cells generated by the original lab and this replication attempt. ## Contents [Figures component][4] contains a visualization of the replication results. These figures are the same as can be found in the Replication Study and include Figure 2 displaying the Western blot results. [Statistical Analyses component][5] contains R scripts that were used for the analyses. [Data Component][6], contains data generated from the experimental work and files used in the analysis and generation of figures, which are in .csv format. [Methods component][7] contains methodology details for this replication attempt which were described in the [Registered Report][8]. [Plasmid quality control data][9] includes the sequencing results from the plasmids used to generate the stable cell lines is available. [Cell line quality control data][10] includes results confirming the cell lines were free of mycoplasma contamination as well as STR DNA profiling of the cell lines used. [1]: [2]: [3]: [4]: [5]: [6]: [7]: [8]: [9]: [10]:
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