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The following data analysis workflow describes the analysis of short read *hsp60* amplicon sequences from environmental samples using the Vib-hspF3-23 and Vib-hspR401-422 primer pair. The associated publication is available at DOI: 10.3389/fmicb.2019.02907. https://www.frontiersin.org/articles/10.3389/fmicb.2019.02907/ ---------- Vib-hspF3-23: **TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG**GAACCCNATGGAYCTKAARCG Vib-hspR401-422: **GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG**GCVATGATHARHAGHGRRCGNG
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