## Registered report: Transcriptional amplification in tumor cells with elevated c-Myc <br> **Abstract:** The [Reproducibility Project: Cancer Biology][1] seeks to address growing concerns about reproducibility in scientific research by conducting replications of 50 papers in the field of cancer biology published between 2010 and 2012. This Registered report describes the proposed replication plan of key experiments from ‘Transcriptional amplification in tumor cells with elevated c-Myc’ by [Lin et al. (2012)][2], published in *Cell* in 2012. The experiments that will be replicated are those reported in Figures 3E and 3F. In these experiments, elevated levels of c-Myc in the P493-6 cell model of Burkitt's lymphoma results in an increase of the total level of RNA using UV/VIS spectrophotometry (Figure 3E; [Lin et al., 2012][3]) and on the mRNA levels/cell for a large set of genes using digital gene expression technology (Figure 3F; [Lin et al., 2012][4]). The Reproducibility Project: Cancer Biology is a collaboration between the [Center for Open Science][5] and [Science Exchange][6], and the results of the replications will be published in *eLife*. ---------- ### Contents This folder contains additional materials relating to the Registered Report for Study 48: Lin et al. 2012, *Cell*. The Registered Report can be found [here][7]. ---------- **Registered Report Initial Draft**: - This document represents the first draft of the selected experimental protocols. It contains the information that the Core team, with help from scientist volunteers, was able to glean from the original publication and its supplemental materials before the original authors were contacted for their input. - <a href="https://osf.io/sg3dz/">Study 48 Registered Report Initial Draft</a> **Power Calculations**: - These documents contain details about how power calculations were performed, and include spreadsheets documenting statistical tests as well as any PRISM files, R scripts or additional materials used. - <a href="https://osf.io/gs5tc/">Study 48 power calculations.xlsx</a> - <a href="https://osf.io/f6qp2/">Custom codesets for NanoString.xlsx </a> - Contains the genes in the custom CodeSet that will be used in the replication study, which combines the unique genes from the three separate custom CodeSets (CS-1, CS-2, and CS-MYC) into one combined set. - <a href="https://osf.io/9wmq8/">Protocol 2 normality homoscedasiticity.R</a> - <a href="https://osf.io/czbg3/">Study 48 1-way Anova Protocol 2.pzfx</a> - <a href="https://osf.io/tixz8/">Protocol 3&4 normality homoscedasiticity.R </a> - <a href="https://osf.io/tj95y/">Protocol3&4.csv</a> - file of original data for importing into R for analysis [1]: https://osf.io/e81xl/wiki/home/ [2]: http://dx.doi.org/10.1016/j.cell.2012.08.026 [3]: http://dx.doi.org/10.1016/j.cell.2012.08.026 [4]: http://dx.doi.org/10.1016/j.cell.2012.08.026 [5]: http://centerforopenscience.org/ [6]: https://www.scienceexchange.com/ [7]: http://elifesciences.org/content/4/e04024