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The replication of Study 36: [Driessens et al., 2012 ][1] was [not pursued][2]; however, this project contains all information pertaining to the attempted replication of key experiments from this paper. It includes the detailed protocols, including reagents and author clarifications where they are available. **Original citation:** Driessens G., Beck B., Caauwe A., Simons B.D., Blanpain C. Defining the mode of tumour growth by clonal analysis. Nature. 2012 Aug 23;488(7412):527-30. doi: 10.1038/nature11344. ---------- Note: This study has been cut from the Reproducibility Project: Cancer Biology due to feasibility constraints in generating the mouse model. [1]: [2]: