**Figure 1. Tissue specific DOX accumulation.** Mice bearing orthotopic 22Rv1 human prostate tumors were intravenously injected with a mixture of PBS, 10 mg/kg DOX and PBS (DOX + PBS), or 10 mg/kg DOX and 4 µmol/kg of iRGD (DOX + iRGD). One hr later tissues were harvested and DOX was quantified by spectrophotometry using absorbance at 490 nm. Tissues from DOX + PBS and DOX + iRGD treated mice [n=4 for both conditions] were examined for DOX accumulation using matched tissues from mice injected with PBS [n=2] as the blank reference samples. DOX accumulation for each tissue was normalized to the mean absorbance of that same tissue type treated with 10 mg/kg DOX and PBS. Means reported and error bars represent s.e.m. Unpaired two-tailed Student's _t_-test between DOX + PBS and DOX + iRGD for prostate tumor tissue; _t_(6) = 0.352, _p_ = .737, with _a priori_ alpha level = .05.
Access [full size image][1] and [R script][2] used to generate this figure. This script will call the [data][3] directly from the OSF.
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**Figure 1 - figure supplement 1.** This is the same experiment as in Figure 1, but with the OD<sub>490</sub> readings plotted for each condition instead of the OD<sub>490</sub> relative to DOX + PBS for each tissue. Mice bearing orthotopic 22Rv1 human prostate tumors were intravenously injected with a mixture of 10 mg/kg DOX and PBS (DOX + PBS), or 10 mg/kg DOX and 4 μmol/kg of iRGD (DOX + iRGD). One hr later tissues were harvested and DOX was quantified by spectrophotometry using absorbance at 490 nm. Means reported and error bars represent s.e.m. Number of mice per condition (n=4).
Access [full size image][4] and [R script][5] used to generate this figure. This script will call the [data][6] directly from the OSF.
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[Statistical Analyses component][7] contains R scripts that were used for the analysis.
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[Additional figures][8] not included in the [manuscript][9] were also generated to provide alternative visualization of the data collected.
[1]: https://osf.io/uf672/
[2]: https://osf.io/b9csz/
[3]: https://osf.io/kp79h/
[4]: https://osf.io/by75p/
[5]: https://osf.io/mpehw/
[6]: https://osf.io/kp79h/
[7]: https://osf.io/u2nwm/wiki/home/
[8]: https://osf.io/zmbrs/wiki/Additional%20figures/
[9]: https://osf.io/tuy3e/
