# Repository overview This repository contains images from the testing of CARD-FISH probes for three general groupings within the *Campylobacteria*: * *Arcobacter* * *Sulfurimonas* * *Sulfurovum* They were used to quantify microbial community composition in samples from the low-temperature hydrothermal vent *Crab Spa* (EPR 9°50'N), and used to determine group-specific carbon fixation in short-term incubations according to the methods described in Dyksma *et al.* (2016). A paper is currently in preparation describing these incubations. The *Sulfurimonas* probe was already used in McNichol et al (2018) to quantify the growth of this taxon in high-pressure incubations. *This paper will be linked here when it is published, or you can contact us for more information.* # If you want to use the probes: You can download the information [here][1]. If the probes are used in any of your publications, kindly cite this repository. Please also note these primers are to be used with the **unlabelled** competitor oligonucleotides (just like a probe but with no fluorophore or enzyme etc) as indicated in the spreadsheet. Also please note that *Sulfurovum* has two probes that can be used together. ## References Dyksma, S., Bischof, K., Fuchs, B. et al. Ubiquitous Gammaproteobacteria dominate dark carbon fixation in coastal sediments. ISME J 10, 1939–1953 (2016). https://doi.org/10.1038/ismej.2015.257 Primary productivity below the seafloor at deep-sea hot springs Jesse McNichol, Hryhoriy Stryhanyuk, Sean P. Sylva, François Thomas, Niculina Musat, Jeffrey S. Seewald, Stefan M. Sievert Proceedings of the National Academy of Sciences Jun 2018, 115 (26) 6756-6761; DOI: https://doi.org/10.1073/pnas.1804351115 ### Other relevant repositories 454 tag sequences from the incubations that are in preparation for publication: https://osf.io/9e6ra/ [1]: https://osf.io/k2gj4/download