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Files within this project are the data for a manuscript at the Journal of Neural Engineering entitled "Chronic monitoring of lower urinary tract activity via a sacral dorsal root ganglia interface". To process the .ns2 files, we recommend using MATLAB 2015b. All neural waveform data was uploaded to Plexon Offline Sorter and saved as .plx files. The timestamps for raw and electrode channels were exported and saved as .txt files. All bladder pressure data and void traces were uploaded on analog channels and saved as .ns2 files. All files are titled under the format "Exp_#_Trial####_mm-dd-yyyy". Impedance files are formated by week rather than by trial. Bladder afferent neural signals were recorded relating to bladder pressure during saline infusion trials. A disposable pressure transducer as well as a fluid infusion line were connected to bladder catheters. Sterile body temperature saline solutions were infused using either programmable syringe pumps or through manual syringe pumping. The saline was infused at a flow fill rate of 2mL/min until leakage or voiding. Most of the LUT signals were well isolated and determined to be SU's (Single units). A few MU's (Multi units) were also found. Electrical stimulation on the pudendal nerve and EMG electrodes was used to determine conduction velocities for stimulation driven units of DRG neurons. Conduction distances were measured during the terminal procedure to be used for velocity calculations. Units with conduction velocities less than 2.5 m/s were considered unmyelinated C-fibers and units with velocities greater than 2.5 m/s were considered myelinated A-fibers. "High velocity" A-fibers were classified as having a velocity greater than 20 m/s. Arrays implanted in the DRG recorded LUT signals after implantation. Neural signals were recorded relating to cutaneous brushing in the perineal region. Each trial was classified based on the point of stimulation. Impedances were tracked during each test session. The acceptable range was set between 10 and 1000 kΩ. Certain test sessions found to have impedances outside of the range were analyzed to see if they contained any stimulation driven units regardless of their unusual impedances. The average impedance for all channels across all experiments was less than 400 kΩ. Voiding trials were either natural or stimulation driven. Natural trials were driven through bladder infusions until leakage or voiding occurred. Stimulation trials were driven through electrical stimulation of the DRG or the pudendal nerve. Certain units were seen to fire during urethral flow and the firing patterns were observed. Awake testing was done with two of the felines (Exp 2, Exp 4). Twice a week each animal was conditioned into a test enclosure prior to the surgery. Trials were performed starting at only a few minutes and extending up to two hours worth of testing once the animal was acclimated to the testing enclosure. The feline were tethered to a fluid infusion line with bladder pressure sensor and the neural recording system. Bladder pressure trials were continuously observed as the feline moved freely about their enclosure, being able to naturally void through grating in the floor. Some .plx files were too large for storage. These files can be provided upon request.
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