Abstract: High-speed atomic force microscopes (HS-AFMs) with high temporal resolution to explore dynamic phenomena at nanoscale resolution. However, HS-AFMs cost much higher than conventional AFMs and there is no open-source HS-AFM controller has been published. The high entry barriers hinder the popularization in both academic and industrial applications of HS-AFMs. Moreover, HS-AFMs generally have small imaging area that limits the fields of application. This study presents an open-source controller that enables a low-cost simplified AFM to achieve a maximum tip-sample velocity of 2,546.5 µm/s (9.3 s/frame, 512×512 pixels), which is nearly 100 times higher than that with an original controller. Moreover, the proposed controller doubles the imaging area of 46.3×46.3 µm, compared to the original system. The low-cost HS-AFM can successfully assess skin barrier function by measuring nano-texture of human skin corneocytes with a constant height DC mode. The entire HS-AFM system costs less than $4,000 that is affordable for resource limited research institutes for high-throughput nanoscale imaging.