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## Registered report: Senescence surveillance of pre-malignant hepatocytes limits liver cancer development <br> **Abstract:** The [Reproducibility Project: Cancer Biology][1] seeks to address growing concerns about reproducibility in scientific research by conducting replications of 50 papers in the field of cancer biology published between 2010 and 2012. This Registered report describes the proposed replication plan of key experiments from ‘Senescence surveillance of pre-malignant hepatocytes limits liver cancer development’ by [Kang et al. (2011)][2], published in *Nature* in 2011. The experiments that will be replicated are those reported in Figures 3B, 3C, 3E, and 4A. In these experiments, [Kang et al. (2011)][3] demonstrate the phenomenon of oncogene-induced cellular senescence and immune-mediated clearance of senescent cells after intrahepatic injection of NRAS (Figures 2I, 3B, 3C, and 3E). Additionally, [Kang et al. (2011)][4] show the specific necessity of CD4+ T cells for immunoclearance of senescent cells (Figure 4A). The Reproducibility Project: Cancer Biology is a collaboration between the [Center for Open Science][5] and [Science Exchange][6], and the results of the replications will be published by [*eLife*][7]. ---------- ### Contents This component contains additional materials relating to the Registered Report for Study 34: Kang et al. 2011, Nature. The Registered Report can be found [here][8]. ---------- **Registered Report Initial Draft**: - This document represents the first draft of the selected experimental protocols. It contains the information that the Core team, with help from scientist volunteers, was able to glean from the original publication and its supplemental materials before the original authors were contacted for their input. - [Study 34 Registered Report Initial Draft][9] **Power Calculations**: - These documents contain details about how power calculations were performed, and include spreadsheets documenting statistical tests as well as any PRISM files, R scripts or additional materials used. - [Study 34 Power calculations.xlsx][10] - [Study 34 2-way ANOVA analysis.pzfx][11] - [Study 34 2-way ANOVAs.R][12] - [Figure 2I:3B 3-way ANOVA.R][13] - [Figure 3C 3-way ANOVA.R][14] - [Figure 3E 3-way ANOVA.R][15] [1]: https://osf.io/e81xl/wiki/home/ [2]: https://doi.org/10.1038/nature10599 [3]: https://doi.org/10.1038/nature10599 [4]: https://doi.org/10.1038/nature10599 [5]: http://centerforopenscience.org/ [6]: https://www.scienceexchange.com/ [7]: https://elifesciences.org/ [8]: https://elifesciences.org/content/4/e04105 [9]: https://osf.io/bdkcq/ [10]: https://osf.io/vpqzs/ [11]: https://osf.io/t6uv8/ [12]: https://osf.io/eqrj4/ [13]: https://osf.io/eztis/ [14]: https://osf.io/wmhi6/ [15]: https://osf.io/6ubvj