**Figure 1. Relative abundance of _Fusobacterium nucleatum_ in colorectal carcinoma versus adjacent normal biopsies.** Representation of quantitative PCR (qPCR) performed independently two times and averaged. The mean relative abundance of _F. nucleatum_ (normalized to _PGT_ expression) in CRC tissue versus adjacent normal tissue of both independent runs is reported for each patient sample and error bars represent _SEM_. The y-axis represents mean fold gene expression change (2<sup>-ΔΔCt</sup>) while the x-axis represents patient samples.
Access [full size image][1] and [R script][2] used to generate this figure. This script will call the data for [run 1][3] and [run 2][4] directly from the OSF.
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**Figure 1 - figure supplement 1. First independent qPCR run.** First of two independent qPCR runs performed in technical duplicate. (**A**) Mean relative abundance of _F. nucleatum_ (normalized to _PGT_ expression) in CRC tissue versus adjacent normal tissue is reported for each patient sample. The y-axis represents mean fold gene expression change (2<sup>-ΔΔCt</sup>) and the x-axis represents patient samples. (**B**) Distribution of raw Ct values for _PGT_ in CRC tumor, adjacent normal tissue, or matched normal samples. (**C**) Distribution of raw Ct values for _F. nucleatum_ in each sample type (CRC tumor, adjacent normal tissue, or matched normal). For graphs of Ct values the y-axis represents a binned count of each Ct score and the x-axis represents the value of Ct scores.
Access [full size image][5] and [R script][6] used to generate this figure. This script will call the [data][7] directly from the OSF.
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**Figure 1 - figure supplement 2. Second independent qPCR run.** Second of two independent qPCR runs performed in technical duplicate. (**A**) Mean relative abundance of _F. nucleatum_ (normalized to _PGT_ expression) in CRC tissue versus adjacent normal tissue is reported for each patient sample. The y-axis represents mean fold gene expression change (2<sup>-ΔΔCt</sup>) and the x-axis represents patient samples. (**B**) Distribution of raw Ct values for _PGT_ in CRC tumor, adjacent normal tissue, or matched normal samples. (**C**) Distribution of raw Ct values for _F. nucleatum_ in each sample type (CRC tumor, adjacent normal tissue, or matched normal). For graphs of Ct values the y-axis represents a binned count of each Ct score and the x-axis represents the value of Ct scores.
Access [full size image][8] and [R script][9] used to generate this figure. This script will call the [data][10] directly from the OSF.
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**Figure 1 - figure supplement 3. Relative abundance of _F. nucleatum_ in colorectal carcinoma versus adjacent normal biopsies in samples with detectable _F. nucleatum_.** Samples in which _F. nucleatum_ was detected in both CRC and adjacent normal tissue were further analyzed (n=7). The mean relative abundance of _F. nucleatum_ (normalized to _PGT_ expression) in CRC tissue versus adjacent normal tissue of both independent runs is reported for each patient sample and error bars represent _SEM_. The y-axis represents mean fold gene expression change (2<sup>-ΔΔCt</sup>) while the x-axis represents patient samples.
Access [full size image][11] and [R script][12] used to generate this figure. This script will call the data for [run 1][3] and [run 2][4] directly from the OSF.
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[Statistical Analyses component][14] contains R scripts that were used for the analysis.
[1]: https://osf.io/6ka4z/
[2]: https://osf.io/umvs2/
[3]: https://osf.io/zwrxm/
[4]: https://osf.io/fz4by/
[5]: https://osf.io/jbk4f/
[6]: https://osf.io/y4hmj/
[7]: https://osf.io/zwrxm/
[8]: https://osf.io/cuxpq/
[9]: https://osf.io/r25ce/
[10]: https://osf.io/fz4by/
[11]: https://osf.io/m2zae/
[12]: https://osf.io/7t5ef/
[13]: https://osf.io/fz4by/
[14]: https://osf.io/9gjyz/wiki/home/
