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Target of replication: *A priori* experimental protocols, materials, and confirmatory analysis plans were peer reviewed, [pre-registered][1], and published by [*eLife*][2]. This component includes all primary and QC data for the first two protocols of the replication of [Vermeulen et al., 2010][3]. These experiments include: ### Contents ### [Figures component][4], contains a visualization of the results. These figures are the same as can be found in the Replication Study and include Figure 1 and Figure 1-figure supplement 1 [Data Component][5], contains data generated from the experimental work related to the first two protocols of the Registered Report (Generation and characterization of colon cancer stem cells) [Methods Component][6], contains the methods relating to the first two protocols of the Registered Report (Generation and characterization of colon cancer stem cells) [1]: https://osf.io/rscpj/ [2]: https://elifesciences.org/articles/07301 [3]: https://www.nature.com/articles/ncb2048 [4]: https://osf.io/e4q5k/wiki/home/ [5]: https://osf.io/7nhkg/wiki/home/ [6]: https://osf.io/qwemb/wiki/home/