## Registered report: The CD47-signal regulated protein alpha (SIRPa) interaction is a therapeutic target for human solid tumors <br> **Abstract:** The [Reproducibility Project: Cancer Biology][1] seeks to address growing concerns about reproducibility in scientific research by conducting replications of 50 papers in the field of cancer biology published between 2010 and 2012. This Registered report describes the proposed replication plan of key experiments from ‘The CD47-signal regulatory protein alpha (SIRPa) interaction is a therapeutic target for human solid tumors’ by [Willingham et al., 2012][2], published in *PNAS* in 2012. The key experiments being replicated are those reported in Figure 6A–C and Table S4. In these experiments, [Willingham et al., 2012][3] test the safety and efficacy of anti-CD47 antibody treatment in immune competent mice utilizing a syngeneic model of mammary tumor growth in FVB mice. The Reproducibility Project: Cancer Biology is a collaboration between the [Center for Open Science][4] and [Science Exchange][5], and the results of the replications will be published in *eLife*. ---------- ### Contents This folder contains additional materials relating to the Registered Report for Study 39: Willingham et al., 2012 (*PNAS*). The published Registered Report can be found [here][6]. **Registered Report Initial Draft**: - This document represents the first draft of the selected experimental protocols. It contains the information that the Core team, with help from scientist volunteers, was able to glean from the original publication and its supplemental materials before the original authors were contacted for their input. - <a href="https://osf.io/rz39d/">Study 39 Registered Report Initial Draft</a> **Power Calculations**: - These documents contain details about how power calculations were performed, and include spreadsheets documenting statistical tests as well as any PRISM files, R scripts or additional materials used. - <a href="https://osf.io/tijzw/">Study 39 Power calculations.xlsx</a> - <a href="https://osf.io/k9bjd/">Protocol 39 normality homoscedasiticity.R</a> - <a href="https://osf.io/wqrus/">Study 39 unequal variance t-tests.R</a> - <a href="https://osf.io/w7us6/">Study 39 Table S4 2 way ANOVA.R</a> - <a href="https://osf.io/79q45/">Study 39 Table S4 MANOVA.R</a> [1]: https://osf.io/e81xl/wiki/home/ [2]: http://www.pnas.org/content/109/17/6662 [3]: http://www.pnas.org/content/109/17/6662 [4]: https://centerforopenscience.org/ [5]: https://www.scienceexchange.com/ [6]: https://elifesciences.org/content/4/e04586