**Poster presented at Association for Psychological Science (APS) Convention 2016.** **Abstract** Psychophysiology tasks are poised to become a major contributor to the NIMH RDoC initiative, but their psychometric properties must first be well understood. This study provides a comprehensive evaluation of the temporal stability, internal consistency, and effect size robustness and stability of three common tasks within the Negative Valence System. **Supporting Summary** The current study provides a comprehensive evaluation of critical psychometric properties of commonly used psychophysiology laboratory tasks and measures within the NIMH RDoC. Participants (N = 128) completed the No Shock, Predictable Shock, Unpredictable Shock (NPU) task, Affective Picture Viewing task, and Resting State task at two study visits separated by one week. We examined potentiation and modulation scores in NPU (predictable or unpredictable shock vs. no shock) and Affective Picture Viewing tasks (pleasant or unpleasant vs. neutral pictures) for startle and corrugator responses with two commonly used quantification methods. We quantified startle potentiation and modulation scores with raw and standardized responses. We quantified corrugator potentiation and modulation in the time and frequency domains. We quantified general startle reactivity in the Resting State Task as the mean raw startle response during the task. For these three tasks, two measures, and two quantification methods we evaluated effect size robustness and stability, internal consistency (i.e., split-half reliability), and one-week temporal stability. The psychometric properties of startle potentiation in the NPU task were good but concerns were noted for corrugator potentiation in this task. Some concerns also were noted for the psychometric properties of both startle and corrugator modulation in the Affective Picture Viewing task, in particular for pleasant picture modulation. Psychometric properties of general startle reactivity in the Resting State task were good. Some salient differences in the psychometric properties of the NPU and Affective Picture Viewing tasks were observed within and across quantification methods. **License** [![Creative Commons License][2]](http://creativecommons.org/licenses/by/4.0/) All components of this project, [Psychometric properties of psychophysiological paradigms in the NIMH RDoC: startle and corrugator response in NPU, affective picture viewing, and resting state tasks](http://dionysus.psych.wisc.edu) by [Jesse T. Kaye, Daniel E. Bradford, and John J. Curtin](http://dionysus.psych.wisc.edu) are licensed under a [Creative Commons Attribution 4.0 International License](http://creativecommons.org/licenses/by/4.0/). <a rel="license" href="http://creativecommons.org/licenses/by/4.0/"><img alt="Creative Commons License" style="border-width:0" src="https://i.creativecommons.org/l/by/4.0/88x31.png" /></a><br /><span xmlns:dct="http://purl.org/dc/terms/" property="dct:title">Psychometric properties of psychophysiological paradigms in the NIMH RDoC: startle and corrugator response in NPU, affective picture viewing, and resting state tasks</span> by <a xmlns:cc="http://creativecommons.org/ns#" href="http://dionysus.psych.wisc.edu/" property="cc:attributionName" rel="cc:attributionURL">Jesse T. Kaye, Daniel E. Bradford, and John J. Curtin </a> is licensed under a <a rel="license" href="http://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution 4.0 International License</a>. [1]: http://dionysus.psych.wisc.edu/WebCMS/self-report-detail.htm?title=Brief_form_Multidimensional_Personality_Questionnaire_%28MPS%29 "lab website" [2]: https://i.creativecommons.org/l/by/4.0/88x31.png