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Assessing the isocitrate-dependent NADPH production and alpha-ketoglutarate-dependent NADPH consumption of wild-type or R172K mutant IDH2  /

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Figure 1. Isocitrate-dependent NADPH production and alpha-ketoglutarate-dependent NADPH consumption of wild-type or R172 mutant IDH2

IDH oxidative and reductive activity assays were performed on lysates of HEK293T cells transfected with wild-type or R172K mutant IDH2, or empty vector. (A) Lysates were assessed for generation of NADPH from NADP+ in the presence of 0.4 mM isocitrate over the indicat…

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