Files within this project contain the data and analysis files for a manuscript entitled, "Pudendal nerve stimulation-driven vaginal blood oscillations."
In order to determine the effects of repeated or prolonged pudendal nerve stimulation on vaginal blood flow, the pudendal nerve of 14 anesthetized female rats were stimulated at various frequencies (1-100 Hz) and durations (0.15-60 minutes), and vaginal blood perfusion was measured with a laser Doppler flowmetry probe. The sheets and MATLAB files presented in this project provide evidence that long-duration pudendal nerve stimulation can elicit maintianed increases in vaginal blood perfusion.
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The following sections are descriptions of the files/folders
1. Experiment Summary Sheet
This sheet contains general surgery information, including weight of rat, type of stimulation, estrous phase, pulse width, measurements taken, etc.
2. MATLAB files
'ReadMe-MATLAB.txt' contains discriptions of the MATLAB files used for data analysis and formation of results.
'ReadMe-OrderOfAnalysis.txt' contains the procedure for proper data analysis.
'expInfo.m' contains experimental stimulation parameters inluding: stimulation/nonstimulation trials, sampling frequency, stimulation frequency, and stimulation amplitudes.
Other individual matlab file functions are described in 'ReadMe-MATLAB.txt'
3. Experiment Data
'ReadMe- Experiment Data Structure.txt' describes how each _LDF.mat and _stim.mat files are organized.
Files Experiment_01 to Experiment_14 contain MATLAB matrices with items described in the 'ReadMe.' Files are named according to the experiment and trial numbers:
Exp#_Trial00##_LDF.mat or Exp#_Trial00##_stim.mat
4. Pudendal Stimulation and Vaginal Blood Perfusion Correlation Sheet
This sheet contains the correlation coefficient, p-value, mean, and standard deviation between ldf and sumstim, neurogenic % and sumstim, and myogenic % and sumstim.
