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Description: The global antibacterial crisis requires urgent attention from environmental engineering and bioengineering. Here, unit operation efficiencies are assessed, in a novel water treatment train capable of remediating antibacterials, metals and DNA. This technological cycle relies on bioremediation, high temperature and pressure. The analyses used 14C-respirometry, spectrometry, and a set of molecular analyses. Multiresistant bacteria hold antibacterial resistance genes (ARGs); they were harnessed for bioremediation of pollutant mixtures. Treatment efficiencies were 25-71% for 8-days aerobic metal reduction and removal (CrVI: 255, Cd: 0.65, and Pb: 0.65 mg L-1 initial concentrations); 34.8% erythromycin (ERY) 20-days biodegradation (from 750 mg L-1). The anaerobic digestion (AD) bioremediated mixed antibacterials (65-73% in 60 days from initial 100 mg L-1). However, high concentrations of mixed antibacterials (SMX+ERY) induced stronger inhibition of enzymatic activity, higher sensitivity of bacteria and acetoclastic methanogens, and higher diversity of ARGs. ARGs justified complete DNA degradation (60°C at 5.8 kPa for 10 min). The suggested coupling sequence of operations was metal then antibacterial aerobic bioremediation (as pre-treatments to anaerobic digestion), anaerobic bioremediation (also yielding biomethane as heat source), recirculation of ARGs in situ, and thermal-barometric DNA degradation.